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CCBY-NC-ND_V3.0_US, Open Access
Journal article
Gene expression profiling in winged and wingless cotton aphids, Aphis gossypii (Hemiptera: Aphididae)
International journal of biological sciences, Vol.10(3), pp.257-267
2014
Handle:
https://hdl.handle.net/2376/115262
PMCID: PMC3957081
PMID: 24644424
Abstract
While trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. In this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, Aphis gossypii, using a tag-based digital gene expression (DGE) approach. Ultra high-throughput Illumina sequencing generated 5.30 and 5.39 million raw tags, respectively, from winged and wingless A. gossypii DGE libraries. We identified 1,663 differentially expressed transcripts, among which 58 were highly expressed in the winged A. gossypii, whereas 1,605 expressed significantly higher in the wingless morphs. Bioinformatics tools, including Gene Ontology, Cluster of Orthologous Groups, euKaryotic Orthologous Groups and Kyoto Encyclopedia of Genes and Genomes pathways, were used to functionally annotate these transcripts. In addition, 20 differentially expressed transcripts detected by DGE were validated by the quantitative real-time PCR. Comparative transcriptomic analysis of sedentary (wingless) and migratory (winged) A. gossyii not only advances our understanding of the trade-offs in wing dimorphic insects, but also provides a candidate molecular target for the genetic control of this agricultural insect pest.
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Details
- Title
- Gene expression profiling in winged and wingless cotton aphids, Aphis gossypii (Hemiptera: Aphididae)
- Creators
- Xiaowei Yang - 1. Department of Entomology, China Agricultural University, Beijing 100193, ChinaXiaoxia Liu - China Agricultural UniversityXiangli Xu - China Agricultural UniversityZhen Li - 1. Department of Entomology, China Agricultural University, Beijing 100193, ChinaYisong Li - 1. Department of Entomology, China Agricultural University, Beijing 100193, ChinaDongyan Song - 2. Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USATian Yu - 2. Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USAFang Zhu - 2. Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USAQingwen Zhang - China Agricultural UniversityXuguo Zhou - University of Kentucky
- Publication Details
- International journal of biological sciences, Vol.10(3), pp.257-267
- Academic Unit
- Department of Entomology
- Publisher
- Australia
- Identifiers
- 99900547335401842
- Language
- English
- Resource Type
- Journal article