Journal article
Gene expression profiling of Puccinia striiformis f. sp. tritici during development reveals a highly dynamic transcriptome
Journal of genetics and genomics, Vol.38(8), pp.357-371
2011
Handle:
https://hdl.handle.net/2376/102682
PMID: 21867962
Abstract
Puccinia striiformis f. sp.
tritici (
Pst) causes stripe rust, one of the most important diseases of wheat worldwide. cDNA libraries had been constructed from urediniospores, germinated urediniospores and haustoria. However, little is known about the expression patterns of the genes related to the infection process and sporulation of the pathogen. In this study, a custom oligonucleotide microarray was constructed using sequences of 442 gene transcripts selected from
Pst cDNA libraries. The expression patterns of the genes were determined by hybridizing the microarray with cDNA from
Pst in vitro and
Pst-infected wheat leaves. The time course study identified 55 transcripts that were differentially expressed during the infection process in a compatible interaction. They were identified to have functions related to the following biological processes, including carbohydrate and lipid metabolism, energy, cell signaling, protein synthesis, cell structure and division. In an incompatible interaction, 17 transcripts of the pathogen were differentially expressed in resistant wheat leaves inoculated with an avirulent
Pst race, ten of which had similar expression patterns to those in the compatible interaction. Several candidates for pathogenicity and virulence/avirulence related genes were also identified. The results of quantitative real-time PCR validated the expression patterns of some selected genes. The study demonstrates that the custom oligonucleotide microarray technology is useful to determine the expression patterns of the pathogen genes involved in different types of the host–pathogen interactions and stages of development.
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Details
- Title
- Gene expression profiling of Puccinia striiformis f. sp. tritici during development reveals a highly dynamic transcriptome
- Creators
- Xueling Huang - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, PR ChinaXianming Chen - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USATristan Coram - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USAMeinan Wang - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USAZhensheng Kang - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, PR China
- Publication Details
- Journal of genetics and genomics, Vol.38(8), pp.357-371
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546527301842
- Language
- English
- Resource Type
- Journal article