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Journal article
Generational comparisons (F1 versus F3) of vinclozolin induced epigenetic transgenerational inheritance of sperm differential DNA methylation regions (epimutations) using MeDIP-Seq
Environmental epigenetics, Vol.3(3), pp.1-12
07/01/2017
Handle:
https://hdl.handle.net/2376/105826
PMCID: PMC5685552
PMID: 29147574
Abstract
Abstract Environmentally induced epigenetic transgenerational inheritance of disease and phenotypic variation has been shown to involve DNA methylation alterations in the germline (e.g. sperm). These differential DNA methylation regions (DMRs) are termed epimutations and in part transmit the transgenerational phenotypes. The agricultural fungicide vinclozolin exposure of a gestating female rat has previously been shown to promote transgenerational disease and epimutations in F3 generation (great-grand-offspring) animals. The current study was designed to investigate the actions of direct fetal exposure on the F1 generation rat sperm DMRs compared to the F3 transgenerational sperm DMRs. A protocol involving methylated DNA immunoprecipitation (MeDIP) followed by next-generation sequencing (Seq) was used in the current study. Bioinformatics analysis of the MeDIP-Seq data was developed and several different variations in the bioinformatic analysis were evaluated. Observations indicate needs to be considered. Interestingly, the F1 generation DMRs were found to be fewer in number and for the most part distinct from the F3 generation epimutations. Observations suggest the direct exposure induced F1 generation sperm DMRs appear to promote in subsequent generations alterations in the germ cell developmental programming that leads to the distinct epimutations in the F3 generation. This may help explain the differences in disease and phenotypes between the direct exposure F1 generation and transgenerational F3 generation. Observations demonstrate a distinction between the direct exposure versus transgenerational epigenetic programming induced by environmental exposures and provide insights into the molecular mechanisms involved in the epigenetic transgenerational inheritance phenomenon.
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Details
- Title
- Generational comparisons (F1 versus F3) of vinclozolin induced epigenetic transgenerational inheritance of sperm differential DNA methylation regions (epimutations) using MeDIP-Seq
- Creators
- Daniel Beck - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USAIngrid Sadler-Riggleman - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USAMichael K Skinner - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA
- Publication Details
- Environmental epigenetics, Vol.3(3), pp.1-12
- Academic Unit
- Biological Sciences, School of
- Publisher
- Oxford Univ Press
- Number of pages
- 12
- Grant note
- NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01ES012974 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Identifiers
- 99900546991801842
- Language
- English
- Resource Type
- Journal article