Journal article
Genetic Diversity and Relatedness of Sweet Cherry (Prunus Avium L.) Cultivars Based on Single Nucleotide Polymorphic Markers
Frontiers in plant science, Vol.3, pp.116-116
06/25/2012
Handle:
https://hdl.handle.net/2376/112666
PMID: 22737155
Abstract
Most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD, and simple sequence repeat (SSR) markers. This study was carried out to assess the utility of single nucleotide polymorphism (SNP) markers generated from 3′ untranslated regions (UTR) for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representing advanced selections, commercial cultivars, and old cultivars imported from different parts of the world were screened with seven SSR markers developed from other
Prunus
species and with 40 SNPs obtained from 3′ UTR sequences of Rainier and Bing sweet cherry cultivars. Both types of marker study had 99 accessions in common. The SSR data was used to validate the SNP results. Results showed that the average number of alleles per locus, mean observed heterozygosity, expected heterozygosity, and polymorphic information content values were higher in SSRs than in SNPs although both set of markers were similar in their grouping of the sweet cherry accessions as shown in the dendrogram. SNPs were able to distinguish sport mutants from their wild type germplasm. For example, “Stella” was separated from “Compact Stella.” This demonstrates the greater power of SNPs for discriminating mutants from their original parents than SSRs. In addition, SNP markers confirmed parentage and also determined relationships of the accessions in a manner consistent with their pedigree relationships. We would recommend the use of 3′ UTR SNPs for genetic fingerprinting, parentage verification, gene mapping, and study of genetic diversity in sweet cherry.
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Details
- Title
- Genetic Diversity and Relatedness of Sweet Cherry (Prunus Avium L.) Cultivars Based on Single Nucleotide Polymorphic Markers
- Creators
- Angel Fernandez i Marti - Departamento Biología Molecular, Parque Científico Tecnológico Aula DeiBlessing Athanson - Irrigated Agriculture Research and Extension Centre, Washington State UniversityTyson Koepke - Department of Horticulture and Landscape Architecture, Washington State UniversityCarolina Font i Forcada - Departamento de Pomología, Estación Experimental de Aula Dei (CSIC)Amit Dhingra - Department of Horticulture and Landscape Architecture, Washington State UniversityNnadozie Oraguzie - Irrigated Agriculture Research and Extension Centre, Washington State University
- Publication Details
- Frontiers in plant science, Vol.3, pp.116-116
- Academic Unit
- Crop and Soil Sciences, Department of; Horticulture, Department of
- Publisher
- Frontiers Research Foundation
- Identifiers
- 99900547605101842
- Language
- English
- Resource Type
- Journal article