Journal article
Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1
Molecular and biochemical parasitology, Vol.69(2), pp.149-159
1995
Handle:
https://hdl.handle.net/2376/107226
PMID: 7770080
Abstract
A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of
Plasmodium spp., Bb-1 is secreted during or just after invasion of host erythrocytes and becomes associated with the cytoplasmic face of the infected cell. The amino terminal sequence of Bb-1 contains a predicted signal peptide and is similar to the amino terminus of another spherical body protein (
BvVA1
225
) which is also translocated to the erythrocyte membrane. Importantly, these two spherical body proteins are the major components of a protective fraction of
B. bovis antigen. There is marked conservation of Bb-1 amino acid sequences and B-lymphocyte epitopes among geographic strains. However, a divergent Bb-1 allele (Bv80) in Australia strains encodes six regions of amino acid polymorphism, including a region of tetrapeptide repeats in the C-terminal half of the polypeptide. Two of the polymorphic regions map to previously defined Th1 epitopes on Bb-1.
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Details
- Title
- Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1
- Creators
- Stephen A Hines - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAGuy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAWendy C Brown - Department of Veterinary Pathobiology, Texas A and M University, College Station, TX, USATerry F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USACarlos E Suarez - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAOdillon Vidotto - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAAllison C Rice-Ficht - Department of Medical Biochemistry and Genetics, Texas A and M University, College Station, TX, USA
- Publication Details
- Molecular and biochemical parasitology, Vol.69(2), pp.149-159
- Academic Unit
- Veterinary Medicine, College of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900547206601842
- Language
- English
- Resource Type
- Journal article