Journal article
Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species
Molecular and biochemical parasitology, Vol.90(1), pp.69-79
1997
Handle:
https://hdl.handle.net/2376/107996
PMID: 9497033
Abstract
Erythrocyte-stage
Babesia equi expresses a 34-kDa immunodominant antigen recognized by antibody from persistently infected horses worldwide. This erythrocyte-stage surface protein, equi merozoite antigen-1 (EMA-1) is encoded by a single copy gene, and was previously shown to share 33% amino acid identity with similar sized proteins of
Theileria sergenti and
T. buffeli. A mean homology of 31% amino acid identity extends to similar sized proteins of
T. parva,
T. annulata and
T. mutans. Genomic and cDNA copies of a second
B. equi gene,
ema2 were cloned. The single copy
ema2 gene encodes a 30-kDa protein (EMA-2) that shares 52% amino acid identity with EMA-1. EMA-2 also shares a mean amino acid identity of 31% with proteins of similar molecular mass from
Theileria species. EMA-1 and EMA-2 each contain a glycosylphosphatidylinositol anchor. These unique erythrocyte-stage surface proteins of
B. equi and
Theileria species lack antigenic repeats, and excluding the signal peptide, contain one or no cysteines. Consistent with the hypothesis that this family of proteins interacts with the erythrocyte surface, the
T. species proteins possess a basic isoelectric point. The
B. equi proteins have acidic isoelectric points, but 24-mer peptides within them have strongly basic net charges.
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Details
- Title
- Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species
- Creators
- D.P Knowles - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-7030, USAL.S Kappmeyer - Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-7030, USAL.E Perryman - Department of Microbiology, Pathology, and Parasitology, North Carolina State University, Raleigh, NC 27606, USA
- Publication Details
- Molecular and biochemical parasitology, Vol.90(1), pp.69-79
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546883201842
- Language
- English
- Resource Type
- Journal article