Genetic and genomewide analysis of simultaneous mutations in acetylated and methylated lysine residues in histone H3 in Saccharomyces cerevisiae

Yi Jin; Amy M Rodriguez; John J Wyrick

doi:10.1534/genetics.108.098897

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Genetic and genomewide analysis of simultaneous mutations in acetylated and methylated lysine residues in histone H3 in Saccharomyces cerevisiae

Journal article

Open access

Peer reviewed

Genetic and genomewide analysis of simultaneous mutations in acetylated and methylated lysine residues in histone H3 in Saccharomyces cerevisiae

Yi Jin, Amy M Rodriguez and John J Wyrick

Genetics (Austin), Vol.181(2), pp.461-472

02/2009

DOI: https://doi.org/10.1534/genetics.108.098897

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https://hdl.handle.net/2376/100617

PMCID: PMC2644940

PMID: 19087956

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Abstract

Gene Expression Regulation, Fungal

Genome-Wide Association Study

Mutagenesis, Site-Directed

Oligonucleotide Array Sequence Analysis

Histones - chemistry

Saccharomyces cerevisiae - genetics

RNA, Messenger - genetics

Gene Expression Profiling

Saccharomyces cerevisiae Proteins - genetics

Saccharomyces cerevisiae - chemistry

Lysine - genetics

Phenotype

Histones - genetics

Genome, Fungal

RNA, Fungal - genetics

Acetylation

Mutation

Lysine - chemistry

Methylation

Saccharomyces cerevisiae - growth & development

Saccharomyces cerevisiae Proteins - chemistry

Telomere - genetics

Acetylated and methylated lysine residues in histone H3 play important roles in regulating yeast gene expression and other cellular processes. Previous studies have suggested that histone H3 acetylated and methylated lysine residues may functionally interact through interdependent pathways to regulate gene transcription. A common genetic test for functional interdependence is to characterize the phenotype of a double mutant. Using this strategy, we tested the genetic interaction between histone H3 mutant alleles that simultaneously eliminate acetylated or methylated lysine residues. Our results indicate that mutation of histone H3 acetylated lysine residues alleviates growth phenotypes exhibited by the H3 methylated lysine mutant. In contrast, histone H3 acetylated and methylated lysine mutants display largely independent effects on yeast gene expression. Intriguingly, these expression changes are preferentially associated with chromosomal regions in which histone H3 lysine residues are hypoacetylated and hypomethylated. Finally, we show that the acetylated and methylated lysine mutants have strikingly different effects on the binding of Sir4 to yeast telomeres, suggesting that histone H3 acetylated lysine residues regulate yeast silencing through a mechanism independent of SIR binding.

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Title: Genetic and genomewide analysis of simultaneous mutations in acetylated and methylated lysine residues in histone H3 in Saccharomyces cerevisiae
Creators: Yi Jin - Center for Reproductive Biology, Washington State University, Pullman, Washington 99164-4660, USA
Amy M Rodriguez
John J Wyrick
Publication Details: Genetics (Austin), Vol.181(2), pp.461-472
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: F32 GM074541 / NIGMS NIH HHS GM074541-01 / NIGMS NIH HHS
Identifiers: 99900546694201842
Language: English
Resource Type: Journal article