Journal article
Genetic and molecular characterization of the maize rp3 rust resistance locus
Genetics (Austin), Vol.162(1), pp.381-394
09/2002
Handle:
https://hdl.handle.net/2376/113975
PMCID: PMC1462242
PMID: 12242248
Abstract
In maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of approximately 140 kb.
Metrics
17 Record Views
Details
- Title
- Genetic and molecular characterization of the maize rp3 rust resistance locus
- Creators
- Craig A Webb - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USATodd E Richter - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USANicholas C Collins - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USAMarie Nicolas - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USAHarold N Trick - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USATony Pryor - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USAScot H Hulbert - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USA
- Publication Details
- Genetics (Austin), Vol.162(1), pp.381-394
- Academic Unit
- Plant Pathology, Department of
- Identifiers
- 99900548100701842
- Language
- English
- Resource Type
- Journal article