Genetic association mapping identifies single nucleotide polymorphisms in genes that affect abscisic acid levels in maize floral tissues during drought

Tim L Setter; Jianbing Yan; Marilyn Warburton; Jean-Marcel Ribaut; Yunbi Xu; Mark Sawkins; Edward S Buckler; Zhiwu Zhang; Michael A Gore

doi:10.1093/jxb/erq308

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Genetic association mapping identifies single nucleotide polymorphisms in genes that affect abscisic acid levels in maize floral tissues during drought

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Genetic association mapping identifies single nucleotide polymorphisms in genes that affect abscisic acid levels in maize floral tissues during drought

Tim L Setter, Jianbing Yan, Marilyn Warburton, Jean-Marcel Ribaut, Yunbi Xu, Mark Sawkins, Edward S Buckler, Zhiwu Zhang and Michael A Gore

Journal of experimental botany, Vol.62(2), pp.701-716

01/2011

DOI: https://doi.org/10.1093/jxb/erq308

Handle:

https://hdl.handle.net/2376/113106

PMCID: PMC3003815

PMID: 21084430

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Abstract

Zea mays - genetics

Flowers - metabolism

Water - metabolism

Molecular Sequence Data

Chromosome Mapping

Phylogeny

Plant Proteins - genetics

Zea mays - classification

Droughts

Gene Expression Regulation, Plant

Abscisic Acid - metabolism

Plant Proteins - metabolism

Polymorphism, Single Nucleotide

Zea mays - metabolism

Flowers - genetics

In maize, water stress at flowering causes loss of kernel set and productivity. While changes in the levels of sugars and abscisic acid (ABA) are thought to play a role in this stress response, the mechanistic basis and genes involved are not known. A candidate gene approach was used with association mapping to identify loci involved in accumulation of carbohydrates and ABA metabolites during stress. A panel of single nucleotide polymorphisms (SNPs) in genes from these metabolic pathways and in genes for reproductive development and stress response was used to genotype 350 tropical and subtropical maize inbred lines that were well watered or water stressed at flowering. Pre-pollination ears, silks, and leaves were analysed for sugars, starch, proline, ABA, ABA-glucose ester, and phaseic acid. ABA and sugar levels in silks and ears were negatively correlated with their growth. Association mapping with 1229 SNPs in 540 candidate genes identified an SNP in the maize homologue of the Arabidopsis MADS-box gene, PISTILLATA, which was significantly associated with phaseic acid in ears of well-watered plants, and an SNP in pyruvate dehydrogenase kinase, a key regulator of carbon flux into respiration, that was associated with silk sugar concentration. An SNP in an aldehyde oxidase gene was significantly associated with ABA levels in silks of water-stressed plants. Given the short range over which decay of linkage disequilibrium occurs in maize, the results indicate that allelic variation in these genes affects ABA and carbohydrate metabolism in floral tissues during drought.

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Title: Genetic association mapping identifies single nucleotide polymorphisms in genes that affect abscisic acid levels in maize floral tissues during drought
Creators: Tim L Setter - Cornell University, Ithaca, NY 14853, USA. TLS1@cornell.edu
Jianbing Yan
Marilyn Warburton
Jean-Marcel Ribaut
Yunbi Xu
Mark Sawkins
Edward S Buckler
Zhiwu Zhang
Michael A Gore
Publication Details: Journal of experimental botany, Vol.62(2), pp.701-716
Academic Unit: Crop and Soil Sciences, Department of
Publisher: England
Identifiers: 99900547538301842
Language: English
Resource Type: Journal article