Journal article
Genetic characterization and molecular mapping of a chlorophyll deficiency gene in sunflower ( Helianthus annuus)
Journal of plant physiology, Vol.166(6), pp.644-651
2009
Handle:
https://hdl.handle.net/2376/101223
PMID: 18947900
Abstract
A major gene controlling chlorophyll deficiency (phenotyped by yellow leaf color,
yl) in sunflower was identified and mapped in an F
2 population derived from a cross between two breeding lines. Greenness degree was scored by a hand-held chlorophyll meter in the F
2 population. Leaf tissue from the parents, F
1 hybrids, and some F
2 progenies were also sampled to determine the chlorophyll content. All F
1 plants had normal green leaf color and the segregation of the plants in the F
2 population fits the monogenic ratio (
χ
(3:1)
2=0.03,
p>0.9), indicating that leaf color is a monogenic trait with normal green dominant over yellow leaf color in this population. The contents of chlorophyll
a, chlorophyll
b, and total chlorophyll in the yellow-leafed lines were reduced by 41.6%, 53.5%, and 44.3%, respectively, in comparison with those in the green-leafed lines. Genetic mapping with molecular markers positioned the gene,
yl, to linkage group 10 of sunflower. An SSR marker, ORS 595, cosegregated with
yl, and a TRAP marker, B26P17ga5-300, was linked to
yl with a genetic distance of 4.2
cM. The molecular marker tightly linked to the chlorophyll deficiency gene will provide insight into the process of chlorophyll metabolism in sunflower.
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Details
- Title
- Genetic characterization and molecular mapping of a chlorophyll deficiency gene in sunflower ( Helianthus annuus)
- Creators
- Bing Yue - Department of Plant Sciences, North Dakota State University, Fargo, ND 58105, USAXiwen Cai - Department of Plant Sciences, North Dakota State University, Fargo, ND 58105, USABrady Vick - US Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, Fargo, ND 58105, USAJinguo Hu - US Department of Agriculture, Agricultural Research Service, Western Regional Plant Introduction Station, Pullman, WA 99164, USA
- Publication Details
- Journal of plant physiology, Vol.166(6), pp.644-651
- Academic Unit
- Agricultural, Human, and Natural Resource Sciences, College of
- Publisher
- Elsevier GmbH
- Identifiers
- 99900546546301842
- Language
- English
- Resource Type
- Journal article