Journal article
Genetic control of mammalian meiotic recombination. I. Variation in exchange frequencies among males from inbred mouse strains
Genetics (Austin), Vol.162(1), pp.297-306
09/2002
Handle:
https://hdl.handle.net/2376/116583
PMCID: PMC1462263
PMID: 12242241
Abstract
Genetic background effects on the frequency of meiotic recombination have long been suspected in mice but never demonstrated in a systematic manner, especially in inbred strains. We used a recently described immunostaining technique to assess meiotic exchange patterns in male mice. We found that among four different inbred strains--CAST/Ei, A/J, C57BL/6, and SPRET/Ei--the mean number of meiotic exchanges per cell and, thus, the recombination rates in these genetic backgrounds were significantly different. These frequencies ranged from a low of 21.5 exchanges in CAST/Ei to a high of 24.9 in SPRET/Ei. We also found that, as expected, these crossover events were nonrandomly distributed and displayed positive interference. However, we found no evidence for significant differences in the patterns of crossover positioning between strains with different exchange frequencies. From our observations of >10,000 autosomal synaptonemal complexes, we conclude that achiasmate bivalents arise in the male mouse at a frequency of 0.1%. Thus, special mechanisms that segregate achiasmate chromosomes are unlikely to be an important component of mammalian male meiosis.
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Details
- Title
- Genetic control of mammalian meiotic recombination. I. Variation in exchange frequencies among males from inbred mouse strains
- Creators
- Kara E Koehler - Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine and the University Hospitals of Cleveland, Cleveland, Ohio 44106-4955, USAJonathan P Cherry - Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine and the University Hospitals of Cleveland, Cleveland, Ohio 44106-4955, USAAudrey Lynn - Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine and the University Hospitals of Cleveland, Cleveland, Ohio 44106-4955, USAPatricia A Hunt - Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine and the University Hospitals of Cleveland, Cleveland, Ohio 44106-4955, USATerry J Hassold - Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine and the University Hospitals of Cleveland, Cleveland, Ohio 44106-4955, USA
- Publication Details
- Genetics (Austin), Vol.162(1), pp.297-306
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900548100201842
- Language
- English
- Resource Type
- Journal article