Journal article
Genetic diversity of 38 spinach (Spinacia oleracea L.) germplasm accessions and 10 commercial hybrids assessed by TRAP markers
Genetic resources and crop evolution, Vol.54(8), pp.1667-1674
12/2007
Handle:
https://hdl.handle.net/2376/104066
Abstract
Target region amplification polymorphism (TRAP) markers were used to assess genetic variability among 38 germplasm accessions and 10 commercial hybrids of spinach (Spinacia oleracea L.), an economically important leafy vegetable crop in many countries. Germplasm accessions with different geographic origins and 10 commercial hybrids were examined. For assessing genetic diversity within accessions, DNA was extracted from 12 individual seedlings from six germplasm accessions and two hybrids. A relatively high level of polymorphism was found within accessions based on 59 polymorphic TRAP markers generated from one fixed primer derived from the Arabidopsis-like telomere repeat sequence and two arbitrary primers. For evaluating interaccession variability, DNA was extracted from a bulk of six to 13 seedlings of each accession. Of the 492 fragments amplified by 12 primer combinations, 96 (19.5%) were polymorphic and discriminated the 48 accessions from each other. The average pair-wise genetic similarity coefficient (Dice) was 57.5% with a range from 23.2 to 85.3%. A dendrogram indicated that the genetic relationships among the accessions were not highly associated with the geographic locations in which the germplasms were collected. The seven commercial hybrids were grouped in three separate clusters, suggesting that the phenotype-based breeding activities tended to reduce the genetic variability. This preliminary study demonstrated that TRAP markers are effective for fingerprinting and evaluating genetic variability among spinach germplasms.
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Details
- Title
- Genetic diversity of 38 spinach (Spinacia oleracea L.) germplasm accessions and 10 commercial hybrids assessed by TRAP markers
- Creators
- Jinguo Hu - USDA-ARS, Northern Crop Science Laboratory 1307 18th Street North Fargo ND 58105-5677 USABeiquan Mou - USDA-ARS 1636 East Alisal Street Salinas CA 93905 USABrady Vick - USDA-ARS, Northern Crop Science Laboratory 1307 18th Street North Fargo ND 58105-5677 USA
- Publication Details
- Genetic resources and crop evolution, Vol.54(8), pp.1667-1674
- Academic Unit
- Agricultural, Human, and Natural Resource Sciences, College of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547000201842
- Language
- English
- Resource Type
- Journal article