Journal article
Genetic immunization with Ehrlichia ruminantium GroEL and GroES homologues
Annals of the New York Academy of Sciences, Vol.969(1), pp.151-154
10/2002
Handle:
https://hdl.handle.net/2376/107397
PMID: 12381581
Abstract
Ehrlichia ruminantium GroEL and GroES genes were amplified from E. ruminantium Welgevonden genomic DNA and were cloned into genetic vaccine and Salmonella expression vectors. These constructs were used to inoculate Balb/c and C57BL/6J mice. Both GroEL and GroES induced low levels of protection in Balb/c and C57BL/6J mice immunized with the Salmonella expression vectors. None of the mice inoculated with the genetic vaccine survived. Immunological memory was also tested in these mice and a correlation between splenocyte proliferation and the survival rate was observed.
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Details
- Title
- Genetic immunization with Ehrlichia ruminantium GroEL and GroES homologues
- Creators
- Alri Pretorius - Molecular Biology Section, Onderstepoort Veterinary Institute, Pretoria, South Africa. Alri@moon.ovi.ac.zaF Van StrijpK A BraytonN E CollinsB A Allsopp
- Publication Details
- Annals of the New York Academy of Sciences, Vol.969(1), pp.151-154
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546992701842
- Language
- English
- Resource Type
- Journal article