Journal article
Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata
Plant molecular biology, Vol.53(4), pp.565-580
11/2003
Handle:
https://hdl.handle.net/2376/112220
PMID: 15010619
Abstract
Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multi-gene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.
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Details
- Title
- Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata
- Creators
- Yan Wang - Intercollege Graduate Degree Program in Plant Physiology, 403 Althouse Laboratory Pennsylvania State University University Park PA 16802 USAXi Wang - Department of Molecular, Cellular and Developmental Biology University of Michigan Ann Arbor MI 48109 USAAndrew McCubbin - Department of Molecular, Cellular and Developmental Biology University of Michigan Ann Arbor MI 48109 USATeh-hui Kao - School of Biological Sciences Washington State University Pullman WA 99164 USA
- Publication Details
- Plant molecular biology, Vol.53(4), pp.565-580
- Academic Unit
- Biological Sciences, School of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547327001842
- Language
- English
- Resource Type
- Journal article