Journal article
Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
Molecular and biochemical parasitology, Vol.179(2), pp.111-113
10/2011
Handle:
https://hdl.handle.net/2376/113302
PMCID: PMC3167272
PMID: 21741414
Abstract
Bovipain-2 (in red), a Babesia bovis cysteine protease, is released into the host erythrocyte. Bovipain-2 is expressed in both virulent and attenuated B. bovis. DAPI stains the nuclei of two B. bovis. [Display omitted]
► Genome sequence of the virulent T2Bo strain reveals 66 peptidases which constitute 2% of the annotated coding sequences. ► Each of the peptidases identified in the virulent parent was represented in the attenuated daughter strain. ► Microarrays analyses of transcript levels for all 66 peptidase-encoding genes were investigated. ► Our study discusses our findings in regards to peptidase involvement in in vivo attenuation of B. bovis.
Identifying virulence determinants in Apicomplexan parasites remains a major gap in knowledge for members within this phylum. We hypothesized that peptidases would segregate with virulence between a virulent parent Babesia bovis strain and an attenuated daughter strain derived by rapid in vivo passage. Using the complete genome sequence of the virulent T2Bo strain, 66 peptidases were identified and active sites confirmed. The presence, sequence identity and expression levels were tested for each of the 66 peptidases in the virulent parent and attenuated daughter T2Bo strains using whole genome, targeted sequencing approaches and microarrays analyses. Quantitative PCR revealed that there was no significant difference in peptidase expression between the virulent and attenuated strains. We conclude that while peptidases may well play a required role in B. bovis pathogenesis, neither loss of peptidase gene content nor reduced gene expression underlies the loss of virulence associated with in vivo passage and attenuation.
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Details
- Title
- Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
- Creators
- Maria Mesplet - Instituto de Patobiología, CICVyA, INTA-Castelar, Buenos Aires, ArgentinaGuy H Palmer - Program of Genomics, Department of Veterinary Microbiology and Pathology and Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, United StatesMonica J Pedroni - Program of Genomics, Department of Veterinary Microbiology and Pathology and Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, United StatesIgnacio Echaide - Estación Experimental Agropecuaria Rafaela, INTA, ArgentinaMonica Florin-Christensen - Instituto de Patobiología, CICVyA, INTA-Castelar, Buenos Aires, ArgentinaLeonhard Schnittger - Instituto de Patobiología, CICVyA, INTA-Castelar, Buenos Aires, ArgentinaAudrey O.T Lau - Program of Genomics, Department of Veterinary Microbiology and Pathology and Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, United States
- Publication Details
- Molecular and biochemical parasitology, Vol.179(2), pp.111-113
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900548058901842
- Language
- English
- Resource Type
- Journal article