Journal article
Genome-wide association mapping of partial resistance to Aphanomyces euteiches in pea
BMC genomics, Vol.17(1), pp.124-124
02/20/2016
Handle:
https://hdl.handle.net/2376/108468
PMCID: PMC4761183
PMID: 26897486
Abstract
Genome-wide association (GWA) mapping has recently emerged as a valuable approach for refining the genetic basis of polygenic resistance to plant diseases, which are increasingly used in integrated strategies for durable crop protection. Aphanomyces euteiches is a soil-borne pathogen of pea and other legumes worldwide, which causes yield-damaging root rot. Linkage mapping studies reported quantitative trait loci (QTL) controlling resistance to A. euteiches in pea. However the confidence intervals (CIs) of these QTL remained large and were often linked to undesirable alleles, which limited their application in breeding. The aim of this study was to use a GWA approach to validate and refine CIs of the previously reported Aphanomyces resistance QTL, as well as identify new resistance loci.
A pea-Aphanomyces collection of 175 pea lines, enriched in germplasm derived from previously studied resistant sources, was evaluated for resistance to A. euteiches in field infested nurseries in nine environments and with two strains in climatic chambers. The collection was genotyped using 13,204 SNPs from the recently developed GenoPea Infinium® BeadChip.
GWA analysis detected a total of 52 QTL of small size-intervals associated with resistance to A. euteiches, using the recently developed Multi-Locus Mixed Model. The analysis validated six of the seven previously reported main Aphanomyces resistance QTL and detected novel resistance loci. It also provided marker haplotypes at 14 consistent QTL regions associated with increased resistance and highlighted accumulation of favourable haplotypes in the most resistant lines. Previous linkages between resistance alleles and undesired late-flowering alleles for dry pea breeding were mostly confirmed, but the linkage between loci controlling resistance and coloured flowers was broken due to the high resolution of the analysis. A high proportion of the putative candidate genes underlying resistance loci encoded stress-related proteins and others suggested that the QTL are involved in diverse functions.
This study provides valuable markers, marker haplotypes and germplasm lines to increase levels of partial resistance to A. euteiches in pea breeding.
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Details
- Title
- Genome-wide association mapping of partial resistance to Aphanomyces euteiches in pea
- Creators
- Aurore Desgroux - INRA, UMR 1347 Agroécologie, 17 rue de Sully, 21065, Dijon Cedex, France. aurore.desgroux@rennes.inra.frVirginie L'Anthoëne - Present Address: Nestlé R&D Center Tours, 101 Avenue Gustave Eiffel, 37097, Tours Cedex 2, France. Virginie.Merot@rdto.nestle.comMartine Roux-Duparque - Present Address: Chambre d'Agriculture de l'Aisne, 1 rue René Blondelle, 02007, Laon Cedex, France. martine.rouxduparque@ma02.orgJean-Philippe Rivière - PISOM, UMT INRA/Terres Inovia, UMR IGEPP 1349, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu Cedex, France. jean-philippe.riviere@rennes.inra.frGrégoire Aubert - INRA, UMR 1347 Agroécologie, 17 rue de Sully, 21065, Dijon Cedex, France. Gregoire.Aubert@dijon.inra.frNadim Tayeh - INRA, UMR 1347 Agroécologie, 17 rue de Sully, 21065, Dijon Cedex, France. Nadim.Tayeh@dijon.inra.frAnne Moussart - Terres Inovia, 11 rue de Monceau, CS 60003, 75378, Paris Cedex, France. Anne.Moussard@rennes.inra.frPierre Mangin - INRA, Domaine Expérimental d'Epoisses, UE0115, 21110, Bretenières Cedex, France. pierre.mangin@dijon.inra.frPierrick Vetel - PISOM, UMT INRA/Terres Inovia, UMR IGEPP 1349, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu Cedex, France. Pierrick.Vetel@rennes.inra.frChristophe Piriou - PISOM, UMT INRA/Terres Inovia, UMR IGEPP 1349, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu Cedex, France. Christophe.Piriou@rennes.inra.frRebecca J McGee - USDA, ARS, Grain Legume Genetics and Physiology Research Unit, Pullman, WA, 99164-6434, USA. Rebecca.McGee@ars.usda.govClarice J Coyne - USDA, ARS, Western Regional Plant Introduction Station, Washington State University, Pullman, WA, 99164-6402, USA. Clarice.Coyne@ars.usda.govJudith Burstin - INRA, UMR 1347 Agroécologie, 17 rue de Sully, 21065, Dijon Cedex, France. judith.burstin@dijon.inra.frAlain Baranger - PISOM, UMT INRA/Terres Inovia, UMR IGEPP 1349, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu Cedex, France. Alain.Baranger@rennes.inra.frMaria Manzanares-Dauleux - AgroCampus Ouest, UMR IGEPP 1349 IGEPP, 65 rue de Saint Brieuc, 35042, Rennes Cedex, France. maria.manzanares@agrocampus-ouest.frVirginie Bourion - INRA, UMR 1347 Agroécologie, 17 rue de Sully, 21065, Dijon Cedex, France. Virginie.Bourion@dijon.inra.frMarie-Laure Pilet-Nayel - PISOM, UMT INRA/Terres Inovia, UMR IGEPP 1349, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu Cedex, France. Marie-Laure.Pilet@rennes.inra.fr
- Publication Details
- BMC genomics, Vol.17(1), pp.124-124
- Academic Unit
- Crop and Soil Sciences, Department of; Horticulture, Department of
- Publisher
- England
- Identifiers
- 99900547692601842
- Language
- English
- Resource Type
- Journal article