Genomic loci and candidate genes underlying inflammatory nociception

Harsha K Nair; Heather Hain; Raymond M Quock; Vivek M Philip; Elissa J Chesler; John K Belknap; William R Lariviere

doi:10.1016/j.pain.2010.11.029

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Genomic loci and candidate genes underlying inflammatory nociception

Journal article

Open access

Peer reviewed

Genomic loci and candidate genes underlying inflammatory nociception

Harsha K Nair, Heather Hain, Raymond M Quock, Vivek M Philip, Elissa J Chesler, John K Belknap and William R Lariviere

Pain (Amsterdam), Vol.152(3), pp.599-606

03/2011

DOI: https://doi.org/10.1016/j.pain.2010.11.029

Handle:

https://hdl.handle.net/2376/106711

PMCID: PMC3039031

PMID: 21195549

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Abstract

Nociception

Inflammation

Quantitative trait locus mapping

Transcript abundance

Acetic acid

Microarray

Heritable genetic factors contribute significantly to inflammatory nociception. To determine candidate genes underlying inflammatory nociception, the current study used a mouse model of abdominal inflammatory pain. BXD recombinant inbred (RI) mouse strains were administered the intraperitoneal acetic acid test, and genome-wide quantitative trait locus (QTL) mapping was performed on the mean number of abdominal contraction and extension movements in 3 distinct groups of BXD RI mouse strains in 2 separate experiments. Combined mapping results detected 2 QTLs on chromosomes (Chr) 3 and 10 across experiments and groups of mice; an additional sex-specific QTL was detected on Chr 16. The results replicate previous findings of a significant QTL, Nociq2, on distal Chr 10 for formalin-induced inflammatory nociception and will aid in identification of the underlying candidate genes. Comparisons of sensitivity to intraperitoneal acetic acid in BXD RI mouse strains with microarray mRNA transcript expression profiles in specific brain areas detected covarying expression of candidate genes that are also found in the detected QTL confidence intervals. The results indicate that common and distinct genetic mechanisms underlie heritable sensitivity to diverse inflammatory insults, and provide a discrete set of high-priority candidate genes to investigate further in rodents and human association studies. Novel genomic regions linked to inflammatory nociception were detected, a previously reported locus was confirmed, and high-priority candidate genes for inflammatory nociception and pain were identified.

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Title: Genomic loci and candidate genes underlying inflammatory nociception
Creators: Harsha K Nair - University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA
Heather Hain - VA Medical Center and Oregon Health & Science University, Portland, OR, USA
Raymond M Quock - Washington State University, Pullman, WA, USA
Vivek M Philip - The Jackson Laboratory, Bar Harbor, ME, USA
Elissa J Chesler - The Jackson Laboratory, Bar Harbor, ME, USA
John K Belknap - VA Medical Center and Oregon Health & Science University, Portland, OR, USA
William R Lariviere - University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA
Publication Details: Pain (Amsterdam), Vol.152(3), pp.599-606
Academic Unit: Psychology, Department of
Publisher: Elsevier B.V
Identifiers: 99900546664001842
Language: English
Resource Type: Journal article