Journal article
Genomic organization and role of SPI-13 in nutritional fitness of Salmonella
International journal of medical microbiology, Vol.308(8), pp.1043-1052
12/2018
Handle:
https://hdl.handle.net/2376/105922
PMID: 30466554
Abstract
Salmonella pathogenicity island 13 (SPI-13) contributes to the virulence of Salmonella. The majority of the SPI-13 genes encode proteins putatively involved in bacterial metabolism, however, their functions largely remain uncharacterized. It is currently unknown if SPI-13 contributes to metabolic fitness of Salmonella and, if so, what are the metabolic substrates for the protein encoded by genes within SPI-13. We employed Phenotype Microarray (Biolog, USA) to compare the metabolic properties of SPI-13 deficient mutant (ΔSPI-13) and the WT parent strain of non-typhoidal Salmonella enterica sub sp. enterica serovar Enteritidis (S. Enteritidis). The results of Phenotype Microarray revealed that SPI-13 is required for efficient utilization of two micronutrients, namely, d-glucuronic acid (DGA) and tyramine (TYR), as sole sources of carbon and/or nitrogen. By systematic deletion of the individual gene(s), we identified specific genes within SPI-13 that are required for efficient utilization of DGA (SEN2977-80) and TYR (SEN2967 and SEN2971-72) as sole nutrient sources. The results show that SPI-13 mediated DGA and TYR metabolic pathways afford nutritional fitness to S. Enteritidis. Comparative genomics analysis of the SPI-13 locus from 247 Salmonella strains belonging to 57 different serovars revealed that SPI-13 genes specifically involved in the metabolism of DGA and TYR are highly conserved in Salmonella enterica. Because DGA and TYR are naturally present as metabolic byproducts in the gastrointestinal tract and other host tissues, we propose a metabolic model that shows that the role of SPI-13 mediated DGA and TYR metabolism in the nutritional fitness of Salmonella is likely linked to nutritional virulence of this pathogen.
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Details
- Title
- Genomic organization and role of SPI-13 in nutritional fitness of Salmonella
- Creators
- Jacob R Elder - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040, USANarayan C Paul - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040, USARaquel Burin - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040, USAJean Guard - Egg Quality and Safety Research Unit, Agriculture Research Service, United StatesDepartment of Agriculture, Athens, GA, 30605, USADevendra H Shah - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 99164-7040, USA
- Publication Details
- International journal of medical microbiology, Vol.308(8), pp.1043-1052
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier GmbH
- Identifiers
- 99900546906201842
- Language
- English
- Resource Type
- Journal article