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Genomic resources for a model in adaptation and speciation research: characterization of the Poecilia mexicana transcriptome1.39 MB
CC BY V4.0, Open Access
Journal article
Genomic resources for a model in adaptation and speciation research: characterization of the Poecilia mexicana transcriptome
BMC genomics, Vol.13(1), pp.652-652
11/21/2012
Handle:
https://hdl.handle.net/2376/108769
PMCID: PMC3585874
PMID: 23170846
Abstract
Background: Elucidating the genomic basis of adaptation and speciation is a major challenge in natural systems with large quantities of environmental and phenotypic data, mostly because of the scarcity of genomic resources for non-model organisms. The Atlantic molly (Poecilia mexicana, Poeciliidae) is a small livebearing fish that has been extensively studied in evolutionary ecology research, particularly because this species has repeatedly colonized extreme environments in the form of caves and toxic hydrogen sulfide containing springs. In such extreme environments, populations show strong patterns of adaptive trait divergence and the emergence of reproductive isolation. Here, we used RNA-sequencing to assemble and annotate the first transcriptome of P. mexicana to facilitate ecological genomics studies in the future and aid the identification of genes underlying adaptation and speciation in the system. Description: We provide the first annotated reference transcriptome of P. mexicana. Our transcriptome shows high congruence with other published fish transcriptomes, including that of the guppy, medaka, zebrafish, and stickleback. Transcriptome annotation uncovered the presence of candidate genes relevant in the study of adaptation to extreme environments. We describe general and oxidative stress response genes as well as genes involved in pathways induced by hypoxia or involved in sulfide metabolism. To facilitate future comparative analyses, we also conducted quantitative comparisons between P. mexicana from different river drainages. 106,524 single nucleotide polymorphisms were detected in our dataset, including potential markers that are putatively fixed across drainages. Furthermore, specimens from different drainages exhibited some consistent differences in gene regulation.
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Details
- Title
- Genomic resources for a model in adaptation and speciation research: characterization of the Poecilia mexicana transcriptome
- Creators
- Joanna L Kelley - Department of Genetics, Stanford University, 300 Pasteur Dr, Stanford, CA, 94305, USACourtney N Passow - Department of Zoology, Oklahoma State University, 501 Life Sciences West, Stillwater, OK, 74078, USAMartin Plath - J.W. Goethe University Frankfurt/M., Biologicum, Evolutionary Ecology Group, Max-von-Laue Str. 13, 60438, Frankfurt am Main, GermanyLenin Arias Rodriguez - División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco (UJAT), C.P. 86150, Villahermosa, Tabasco, MexicoMuh-Ching Yee - Department of Genetics, Stanford University, 300 Pasteur Dr, Stanford, CA, 94305, USAMichael Tobler - Department of Zoology, Oklahoma State University, 501 Life Sciences West, Stillwater, OK, 74078, USA
- Publication Details
- BMC genomics, Vol.13(1), pp.652-652
- Academic Unit
- Kelley Lab; Biological Sciences, School of
- Publisher
- BioMed Central
- Identifiers
- 99900546803201842
- Language
- English
- Resource Type
- Journal article