Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia

Chiemi Miura; Yosuke Shimizu; Maho Uehara; Yuichi Ozaki; Graham Young; Takeshi Miura

doi:10.1530/REP-11-0203

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Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia

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Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia

Chiemi Miura, Yosuke Shimizu, Maho Uehara, Yuichi Ozaki, Graham Young and Takeshi Miura

Reproduction (Cambridge, England), Vol.142(6), pp.869-877

12/2011

DOI: https://doi.org/10.1530/REP-11-0203

Handle:

https://hdl.handle.net/2376/106689

PMID: 21976616

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Abstract

Dihydrotestosterone - analogs & derivatives

Gonadal Steroid Hormones - metabolism

Receptors, Somatotropin - metabolism

Testis - metabolism

Cell Proliferation

Growth Hormone - metabolism

Spermatogenesis

Anguilla - metabolism

Male

Recombinant Proteins

Testosterone - analogs & derivatives

RNA, Messenger - metabolism

Spermatogonia - physiology

Animals

Insulin-Like Growth Factor I - metabolism

Gh plays important roles in development, somatic growth and gametogenesis in vertebrates. To determine the physiological role of Gh in reproduction in male teleosts, the expression of genes encoding Gh and the two Gh receptors (Ghrs) during spermatogenesis, and the action of Gh in vitro was examined using the Japanese eel (Anguilla japonica). gh, ghr1 and ghr2 mRNA transcripts were detected in all spermatogenic stages. In situ hybridization showed the presence of ghr1 and ghr2 mRNA in the germ cells. Immunohistochemistry using an antiserum against eel Gh indicated that Gh protein was localized to Sertoli cells surrounding the germ cells in early spermatogenesis. Recombinant eel Gh induced spermatogonial proliferation in a testis organ culture system, an effect that was independent from the production of steroid hormones or Igf1. This study identifies a role for eel Gh in the regulation of early spermatogenesis, particularly in the mitotic phase of spermatogenesis, that is not mediated by either steroid hormones or Igf1 production.

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Title: Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia
Creators: Chiemi Miura - Research Group for Reproductive Physiology, South Ehime Fisheries Research Center, Ehime University, Funakoshi, Ainan, Japan
Yosuke Shimizu
Maho Uehara
Yuichi Ozaki
Graham Young
Takeshi Miura
Publication Details: Reproduction (Cambridge, England), Vol.142(6), pp.869-877
Academic Unit: UNKNOWN
Publisher: England
Identifiers: 99900546900701842
Language: English
Resource Type: Journal article