Glial-derived neurotrophic factor promotes ovarian primordial follicle development and cell-cell interactions during folliculogenesis

Gretchen Dole; Eric E Nilsson; Michael K Skinner

doi:10.1530/REP-07-0405

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Glial-derived neurotrophic factor promotes ovarian primordial follicle development and cell-cell interactions during folliculogenesis

Journal article

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Glial-derived neurotrophic factor promotes ovarian primordial follicle development and cell-cell interactions during folliculogenesis

Gretchen Dole, Eric E Nilsson and Michael K Skinner

Reproduction (Cambridge, England), Vol.135(5), pp.671-682

05/2008

DOI: https://doi.org/10.1530/REP-07-0405

Handle:

https://hdl.handle.net/2376/110391

PMCID: PMC5703190

PMID: 18304989

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Abstract

Oligonucleotide Array Sequence Analysis

Ovarian Follicle - growth & development

Ovary - chemistry

Glial Cell Line-Derived Neurotrophic Factor Receptors - metabolism

Gene Expression Profiling - methods

Ovary - cytology

Reverse Transcriptase Polymerase Chain Reaction

Rats, Sprague-Dawley

Ovarian Follicle - chemistry

Ovarian Follicle - drug effects

Animals

Glial Cell Line-Derived Neurotrophic Factor - pharmacology

Stem Cell Factor - pharmacology

Cell Communication - drug effects

Transcription, Genetic

Glial Cell Line-Derived Neurotrophic Factor Receptors - analysis

Organ Culture Techniques

Computational Biology

Immunohistochemistry

Female fertility is determined in part by the size and development of the primordial follicle pool. The current study investigates the role of glial cell-line-derived neurotrophic factor (GDNF) in the regulation of primordial follicle development in the ovary. Ovaries from 4-day-old female rat pups were maintained in organ culture for 10 days in the absence (control) or presence of GDNF or kit ligand (KL)/stem cell factor. Ovaries treated with GDNF contained a significant increase in developing follicles, similar to that observed with KL treatment previously shown to promote follicle development. The actions of GDNF on the ovarian transcriptome were investigated with a microarray analysis. Immunohistochemical studies demonstrated that GDNF is localized to oocyte cytoplasm in follicles of all developmental stages, as well as to cumulus granulosa cells and theca cells in antral follicles. GDNF receptor alpha1 (GFRalpha1) staining was localized to oocyte cytoplasm of primordial and primary follicles, and at reduced levels in the oocytes of antral follicles. GFRalpha1 was present in mural granulosa cells of antral follicles, theca cells, and ovarian surface epithelium. The localization studies were confirmed with molecular analysis. Microarray analysis was used to identify changes in the ovarian transcriptome and further elucidate the signaling network regulating early follicle development. Observations indicate that GDNF promotes primordial follicle development and mediates autocrine and paracrine cell-cell interactions required during folliculogenesis. In contrast to the testis, ovarian GDNF is predominantly produced by germ cells (oocytes) rather than somatic cells.

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Title: Glial-derived neurotrophic factor promotes ovarian primordial follicle development and cell-cell interactions during folliculogenesis
Creators: Gretchen Dole - School of Molecular Biosciences, Center for Reproductive Biology, Washington State University, Pullman, Washington 99164-4231, USA
Eric E Nilsson
Michael K Skinner
Publication Details: Reproduction (Cambridge, England), Vol.135(5), pp.671-682
Academic Unit: Biological Sciences, School of
Publisher: England
Grant note: R01 HD048898 / NICHD NIH HHS
Identifiers: 99900547257701842
Language: English
Resource Type: Journal article