Global gene expression in the human fetal testis and ovary

Brenda Houmard; Christopher Small; Lizhong Yang; Theresa Naluai-Cecchini; Edith Cheng; Terry Hassold; Michael Griswold

doi:10.1095/biolreprod.108.075747

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Global gene expression in the human fetal testis and ovary

Journal article

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Peer reviewed

Global gene expression in the human fetal testis and ovary

Brenda Houmard, Christopher Small, Lizhong Yang, Theresa Naluai-Cecchini, Edith Cheng, Terry Hassold and Michael Griswold

Biology of reproduction, Vol.81(2), pp.438-443

08/2009

DOI: https://doi.org/10.1095/biolreprod.108.075747

Handle:

https://hdl.handle.net/2376/104659

PMID: 19369649

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Abstract

Testis - metabolism

Oligonucleotide Array Sequence Analysis

Ovary - embryology

Humans

Databases, Genetic

Male

Gene Expression Profiling

Sex Characteristics

RNA, Messenger - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Gestational Age

Pregnancy

Meiosis - genetics

Phenotype

Sex Determination Processes

Analysis of Variance

Testis - embryology

Models, Biological

Gene Expression - physiology

Female

Software

Ovary - metabolism

This study describes a temporal profile of gene expression from normal human fetal testes and ovaries. Gonads from 34 fetuses between 9 wk and 20 wk of gestation were obtained from the Department of Pathology and the Birth Defects Research Laboratory at the University of Washington. Relative transcript levels were determined using the Affymetrix Human Genome U133A Plus 2.0 arrays. Sex determination occurs in the human gonad at approximately 6 wk of gestation with development of the testis driven by expression of SRY. In this study, SRY transcript was present and elevated at 9 wk of gestation in the testis but was absent in the ovary. The transcript levels of other testis-specific factors SOX9 and AMH and the steroidogenic genes CYP17A1, CYP11A1, STAR, and HSD17B3 were all significantly higher in the testis. In contrast, transcripts known to be involved in meiosis, including STRA8, SPO11, SYCP3, TEX11, TEX14, and STAG3, showed highest expression in the fetal ovary beginning at Week 12. These gene expression profiles will be a resource for understanding and defining normal gonad development and provide the opportunity to dissect abnormal development.

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Title: Global gene expression in the human fetal testis and ovary
Creators: Brenda Houmard - Department of Obstetrics & Gynecology, University of Washington, Seattle, Washington, USA
Christopher Small
Lizhong Yang
Theresa Naluai-Cecchini
Edith Cheng
Terry Hassold
Michael Griswold
Publication Details: Biology of reproduction, Vol.81(2), pp.438-443
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R37 HD010808 / NICHD NIH HHS R01 HD21341 / NICHD NIH HHS HD 10808 / NICHD NIH HHS U54 HD042454 / NICHD NIH HHS R01 HD010808 / NICHD NIH HHS R01 HD021341 / NICHD NIH HHS U54 HD 42454 / NICHD NIH HHS
Identifiers: 99900546675701842
Language: English
Resource Type: Journal article