Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9

Ramji K Bhandari; Md M Haque; Michael K Skinner

doi:10.1371/journal.pone.0043380

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Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9

Journal article

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Peer reviewed

Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9

Ramji K Bhandari, Md M Haque and Michael K Skinner

PloS one, Vol.7(9), pp.e43380-e43380

2012

DOI: https://doi.org/10.1371/journal.pone.0043380

Handle:

https://hdl.handle.net/2376/107700

PMCID: PMC3440412

PMID: 22984422

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Abstract

SOX9 Transcription Factor - metabolism

Protein Binding - genetics

Reproducibility of Results

Testis - metabolism

Gene Regulatory Networks - genetics

Nucleotide Motifs - genetics

Sex Determination Processes - genetics

Genome - genetics

Molecular Sequence Data

Gene Expression Profiling

Signal Transduction - genetics

Promoter Regions, Genetic - genetics

Rats, Sprague-Dawley

Animals

Chromatin Immunoprecipitation

Gene Expression Regulation, Developmental

Base Sequence

Sex-Determining Region Y Protein - metabolism

Polymerase Chain Reaction

A major event in mammalian male sex determination is the induction of the testis determining factor Sry and its downstream gene Sox9. The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Sertoli cell differentiation and testis development. A modified ChIP-Chip analysis using a comparative hybridization was used to identify 71 direct downstream binding targets for SRY and 109 binding targets for SOX9. Interestingly, only 5 gene targets overlapped between SRY and SOX9. In addition to the direct response element binding gene targets, a large number of atypical binding gene targets were identified for both SRY and SOX9. Bioinformatic analysis of the downstream binding targets identified gene networks and cellular pathways potentially involved in the induction of Sertoli cell differentiation and testis development. The specific DNA sequence binding site motifs for both SRY and SOX9 were identified. Observations provide insights into the molecular control of male gonadal sex determination.

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Title: Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9
Creators: Ramji K Bhandari - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, Washington, United States of America
Md M Haque
Michael K Skinner
Publication Details: PloS one, Vol.7(9), pp.e43380-e43380
Academic Unit: Biological Sciences, School of
Publisher: Public Library Science; United States
Number of pages: 17
Grant note: United States National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
Identifiers: 99900546922401842
Language: English
Resource Type: Journal article