Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector

G Kenitra Hammac; Sebastián Aguilar Pierlé; Xiaoya Cheng; Glen A Scoles; Kelly A Brayton

doi:10.1186/1756-3305-7-193

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Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector

Journal article

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Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector

G Kenitra Hammac, Sebastián Aguilar Pierlé, Xiaoya Cheng, Glen A Scoles and Kelly A Brayton

Parasites & vectors, Vol.7(1), pp.193-193

04/21/2014

DOI: https://doi.org/10.1186/1756-3305-7-193

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https://hdl.handle.net/2376/101046

PMCID: PMC4022386

PMID: 24751137

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https://doi.org/10.1186/1756-3305-7-193View

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Abstract

Dermacentor - microbiology

Anaplasma marginale - physiology

Animals

Membrane Proteins - genetics

Bacterial Proteins - genetics

Bacterial Proteins - metabolism

Male

Transcription, Genetic

Membrane Proteins - metabolism

Gene Expression Regulation, Bacterial - physiology

Pathogens dependent upon vectors for transmission to new hosts undergo environment specific changes in gene transcription dependent on whether they are replicating in the vector or the mammalian host. Differential gene transcription, especially of potential vaccine candidates, is of interest in Anaplasma marginale, the tick-borne causative agent of bovine anaplasmosis. RNA-seq technology allowed a comprehensive analysis of the transcriptional status of A. marginale genes in two conditions: bovine host blood and tick derived cell culture, a model for the tick vector. Quantitative PCR was used to assess transcription of a set of genes in A. marginale infected tick midguts and salivary glands at two time points during the transmission cycle. Genes belonging to fourteen pathways or component groups were found to be differentially transcribed in A. marginale in the bovine host versus the tick vector. One of the most significantly altered groups was composed of surface proteins. Of the 56 genes included in the surface protein group, eight were up regulated and 26 were down regulated. The down regulated surface protein encoding genes include several that are well studied due to their immunogenicity and function. Quantitative PCR of a set of genes demonstrated that transcription in tick cell culture most closely approximates transcription in salivary glands of recently infected ticks. The ISE6 tick cell culture line is an acceptable model for early infection in tick salivary glands, and reveals disproportionate down regulation of surface protein genes in the tick. Transcriptional profiling in other cell lines may help us simulate additional microenvironments. Understanding vector-specific alteration of gene transcription, especially of surface protein encoding genes, may aid in the development of vaccines or transmission blocking therapies.

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Title: Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
Creators: G Kenitra Hammac
Sebastián Aguilar Pierlé
Xiaoya Cheng
Glen A Scoles
Kelly A Brayton - Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G, Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USA. kbrayton@vetmed.wsu.edu
Publication Details: Parasites & vectors, Vol.7(1), pp.193-193
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: England
Grant note: T32-AI0070 / NIAID NIH HHS AI44005 / NIAID NIH HHS
Identifiers: 99900546627201842
Language: English
Resource Type: Journal article