Glucuronidation of trans-3'-hydroxycotinine by UGT2B17 and UGT2B10

Gang Chen; Nino E Giambrone; Philip Lazarus

doi:10.1097/FPC.0b013e32834ff3a5

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Glucuronidation of trans-3'-hydroxycotinine by UGT2B17 and UGT2B10

Journal article

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Glucuronidation of trans-3'-hydroxycotinine by UGT2B17 and UGT2B10

Gang Chen, Nino E Giambrone and Philip Lazarus

Pharmacogenetics and genomics, Vol.22(3), pp.183-190

03/2012

DOI: https://doi.org/10.1097/FPC.0b013e32834ff3a5

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https://hdl.handle.net/2376/100930

PMCID: PMC3275669

PMID: 22228205

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Abstract

Cotinine - analogs & derivatives

Genetic Association Studies

Minor Histocompatibility Antigens

Humans

Male

Polymorphism, Genetic

Glucuronosyltransferase - genetics

Smoking - metabolism

Glucuronosyltransferase - metabolism

Cotinine - metabolism

Gene Deletion

HEK293 Cells

Amino Acid Substitution - genetics

Female

Microsomes, Liver - enzymology

Kinetics

Trans-3'-Hydroxycotinine (3HC) and its glucuronide are major nicotine metabolites excreted in the urine of smokers and other tobacco users. Although several members of the UDP-glucuronosyltransferase (UGT) family of enzymes were previously shown to be active in catalyzing the formation of 3HC and its glucuronide, a comprehensive screening of all known human UGT1A and 2B enzymes for glucuronidation activity against 3HC was not previously performed. In the present study, human liver microsomes (HLM), eight UGT1A and six UGT2B enzymes were screened for activity against 3HC. UGT2B17 exhibited the highest O-glucuronidation activity, exhibiting a four-fold lower (P<0.005) KM (8.3 mmol/l) compared with that observed for UGTs 1A9 (35 mmol/l) or 2B7 (31 mmol/l) and a KM smaller compared with that observed for human liver microsomes (HLM; 26 mmol/l). The KM for 3HC-O-Gluc formation was 3.1-fold lower (P<0.0005) in HLM from male participants exhibiting the wild-type genotype UGT2B17 (*1/*1) compared with that in HLM from participants homozygous for the UGT2B17 deletion genotype [UGT2B17 (*2/*2)]. Both UGTs 2B10 and 1A4 exhibited 3HC-N-Gluc formation activity, with UGT2B10 exhibiting a four-fold lower (P<0.05) KM (13 mmol/l) compared with that observed for UGT1A4 (57 mmol/l) and, which was similar to the KM observed in HLM (14 mmol/l). There was 91 (P<0.0001) and 39% (P<0.001) decreases in the 3HC-N-Gluc formation activities in HLM from participants with the UGT2B10 (*2/*2) and UGT2B10 (*1/*2) genotypes, respectively, compared with that of HLM from participants with the wild-type UGT2B10 (*1/*1) genotype. These results suggest that UGT2B17 and UGT2B10 play key roles in the glucuronidation of 3HC in the human liver and that functional polymorphisms in UGT2B17 and UGT2B10 are associated with significantly reduced glucuronidation activities against 3HC.

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Title: Glucuronidation of trans-3'-hydroxycotinine by UGT2B17 and UGT2B10
Creators: Gang Chen - Departments of Public Health Sciences, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
Nino E Giambrone
Philip Lazarus
Publication Details: Pharmacogenetics and genomics, Vol.22(3), pp.183-190
Academic Unit: Pharmaceutical Sciences, Department of
Publisher: United States
Grant note: R01 DE013158-08 / NIDCR NIH HHS R01 DE013158 / NIDCR NIH HHS R01 DE013158-10 / NIDCR NIH HHS R01 DE013158-07A2 / NIDCR NIH HHS R01 DE013158-11 / NIDCR NIH HHS R01-DE13158 / NIDCR NIH HHS R01 DE013158-09 / NIDCR NIH HHS
Identifiers: 99900546796501842
Language: English
Resource Type: Journal article