Journal article
Glycoprotein D Receptor-Dependent, Low-pH-Independent Endocytic Entry of Herpes Simplex Virus Type 1
Journal of virology, Vol.79(11), pp.6655-6663
06/01/2005
Handle:
https://hdl.handle.net/2376/107694
PMCID: PMC1112142
PMID: 15890903
Abstract
ABSTRACT Two herpes simplex virus type 1 (HSV-1) entry pathways have been described: direct fusion between the virion envelope and the plasma membrane, as seen on Vero cells, and low-pH-dependent endocytosis, as seen on CHO nectin-1 and HeLa cells. In this paper, we studied HSV entry into C10 murine melanoma cells and identified a third entry pathway for this virus. During entry into C10 cells, virion envelope glycoproteins rapidly became protected from the membrane-impermeable chemical cross-linker BS3 and from proteinase K. Protection was gD receptor dependent, and the time taken to detect protected protein was proportional to the rate of virus entry. Ultrastructural examination revealed that virions attached to the surface of C10 cells were localized to membrane invaginations, whereas those on the surface of receptor-negative B78 cells were peripherally attached. Virus entry into C10 cells was energy dependent, and intracellular enveloped virions were seen within membrane-bound vesicles consistent with endocytic entry. Entry was not inhibited by bafilomycin A1 or ammonium chloride, showing that passage of the virion through a low-pH environment was not required for infection. Resistance to similar reagents should therefore not be taken as proof of HSV entry by a nonendosomal pathway. These data define a novel gD receptor-dependent acid-independent endocytic entry pathway for HSV.
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Details
- Title
- Glycoprotein D Receptor-Dependent, Low-pH-Independent Endocytic Entry of Herpes Simplex Virus Type 1
- Creators
- Richard S. B Milne - Department of Microbiology, Center for Oral Health Research, School of Dental Medicine, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104Anthony V Nicola - Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-1888J. Charles Whitbeck - Department of Microbiology, Center for Oral Health Research, School of Dental Medicine, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104Roselyn J Eisenberg - Center for Oral Health Research, School of Dental Medicine, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104Gary H Cohen - Department of Microbiology, Center for Oral Health Research, School of Dental Medicine
- Publication Details
- Journal of virology, Vol.79(11), pp.6655-6663
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Identifiers
- 99900547190301842
- Language
- English
- Resource Type
- Journal article