Journal article
Graphene Quantum Dot-MnO2 Nanosheet Based Optical Sensing Platform: A Sensitive Fluorescence "Turn Off-On" Nanosensor for Glutathione Detection and Intracellular Imaging
ACS applied materials & interfaces, Vol.8(34), pp.21990-21996
08/31/2016
PMID: 27494553
Abstract
Glutathione (GSH) monitoring has attracted extensive attention because it serves a vital role in human pathologies. Herein, a convenient fluorescence "turn off-on" nanosensor based on graphene quantum dots (GQDs)-manganese dioxide (MnO2) nanosheet has been designed for selective detection of GSH in living cells. The fluorescence intensity of GQDs can be quenched by MnO2 nanosheets via a fluorescence resonance energy transfer. However, GSH can reduce MnO2 nanosheets to Mn2+ cations and release GQDs, causing sufficient recovery of fluorescent signal. The MnO2 nanosheets serve as both fluorescence nanoquencher and GSH recognizer in the sensing platform. The sensing platform displayed a sensitive response to GSH in the range of 0.5-10 mu mol L-1, with a detection limit of 150 nmol L-1. Furthermore, the chemical response of the GQDs-MnO2 nanoprobe exhibits high selectivity toward GSH over other electrolytes and biomolecules. Most importantly, the promising platform was successfully applied in monitoring the intracellular GSH in living cells, indicating its great potential to be used in disease diagnosis. Meanwhile, this GQDs-MnO2 platform is also generalizable and can be easily expanded to the detection and imaging of other reactive species in living cells.
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Details
- Title
- Graphene Quantum Dot-MnO2 Nanosheet Based Optical Sensing Platform: A Sensitive Fluorescence "Turn Off-On" Nanosensor for Glutathione Detection and Intracellular Imaging
- Creators
- Xu Yan - Washington State UniversityYang Song - Jilin UniversityChengzhou Zhu - Jilin UniversityJunhua Song - Jilin UniversityDan Du - Washington State UniversityXingguang Su - Jilin UniversityYuehe Lin - Washington State University
- Publication Details
- ACS applied materials & interfaces, Vol.8(34), pp.21990-21996
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Chemical Soc
- Number of pages
- 7
- Grant note
- 21575047 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) China Scholarship Council (CSC); China Scholarship Council WSU
- Identifiers
- 99901227839101842
- Language
- English
- Resource Type
- Journal article