Journal article
Graphene-based immunosensor for electrochemical quantification of phosphorylated p53 (S15)
Analytica chimica acta, Vol.699(1), pp.44-48
2011
Handle:
https://hdl.handle.net/2376/113527
PMID: 21704756
Abstract
A new graphene-based immunosensor for electrochemical quantification of phosphorylated p53 on serine 15 (phospho-p5315) was proposed. This easily fabricated immunosensor provides a potential tool for analysis of other biomarkers. ► A new graphene-based immunosensor for electrochemical quantification of phosphorylated p53 on serine 15 (phospho-p53 15) was proposed. ► Graphene served as sensor platform greatly increased detection sensitivity. ► This easily fabricated immunosensor provides a potential tool for analysis of other biomarkers. We reported a graphene-based immunosensor for electrochemical quantification of phosphorylated p53 on serine 15 (phospho-p53 15), a potential biomarker of gamma-radiation exposure. The principle is based on sandwich immunoassay and the resulting immunocomplex is formed among phospho-p53 capture antibody, phospho-p53 15 antigen, biotinylated phospho-p53 15 detection antibody and horseradish peroxidase (HRP)-labeled streptavidin. The introduced HRP results in an electrocatalytic response to reduction of hydrogen peroxide in the presence of thionine. Graphene served as sensor platform not only promotes electron transfer, but also increases the surface area to introduce a large amount of capture antibody, thus increasing the detection sensitivity. The experimental conditions including blocking agent, immunoreaction time and substrate concentration have been optimized. Under the optimum conditions, the increase of response current is proportional to the phospho-p53 15 concentration in the range of 0.2–10 ng mL −1, with the detection limit of 0.1 ng mL −1. The developed immunosensor exhibits acceptable stability and reproducibility and the assay results for phospho-p53 15 are in good correlation with the known values. This easily fabricated immunosensor provides a new promising tool for analysis of phospho-p53 15 and other phosphorylated proteins.
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Details
- Title
- Graphene-based immunosensor for electrochemical quantification of phosphorylated p53 (S15)
- Creators
- Yunying Xie - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaAiqiong Chen - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaDan Du - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaYuehe Lin - Pacific Northwest National Laboratory, Richland, WA 99352 USA
- Publication Details
- Analytica chimica acta, Vol.699(1), pp.44-48
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547940101842
- Language
- English
- Resource Type
- Journal article