Journal article
Growth hormone-releasing hormone antibodies suppress sleep and prevent enhancement of sleep after sleep deprivation
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.263(5), pp.R1078-R1085
11/01/1992
Handle:
https://hdl.handle.net/2376/113958
PMID: 1443226
Abstract
Previous reports suggest that the hypothalamic growth hormone-releasing hormone (GHRH) promotes sleep, especially non-rapid-eye-movement sleep (NREMS). To evaluate the role of endogenous GHRH in sleep regulation, the effects of antibodies to rat GHRH (GHRH-ab) were studied on normal sleep, brain temperature (Tbr), and GH secretion in experiment I and on enhanced sleep after sleep deprivation in experiment II. In experiment I, affinity-purified GHRH-ab (50 and 200 micrograms) raised in goats and a control goat immunoglobulin G (IgG) preparation were injected intracerebroventricularly (icv) in rats 1 h before the onset of the light cycle, and sleep-wake activity and Tbr were recorded for the next 12 or 23 h. Both doses of GHRH-ab suppressed NREMS and REMS throughout the light cycle. Sleep durations at night were normal. Electroencephalographic (EEG) slow-wave activity, characterized by EEG slow-wave amplitudes, was reduced after GHRH-ab during both the light and the dark cycles. Plasma GH concentrations measured 6-12 h after injection of GHRH-ab (200 micrograms) were diminished. Both the control IgG and GHRH-ab elicited fever. In experiment II, the sleep-wake activity and Tbr of rats were recorded for 24 h in three experimental conditions: base-line with icv injection of IgG, 3-h sleep deprivation with icv IgG injection, and 3-h sleep deprivation with icv GHRH-ab (200 micrograms). After sleep deprivation (+IgG), a prompt increase in EEG slow-wave activity (power density analysis) and late increases in NREMS and REMS durations were found.
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Details
- Title
- Growth hormone-releasing hormone antibodies suppress sleep and prevent enhancement of sleep after sleep deprivation
- Creators
- F Obal - Department of Physiology and Biophysics, University of Tennessee,Memphis 38163L Payne - Department of Physiology and Biophysics, University of Tennessee,Memphis 38163M Opp - Department of Physiology and Biophysics, University of Tennessee,Memphis 38163P Alfoldi - Department of Physiology and Biophysics, University of Tennessee,Memphis 38163L Kapas - Department of Physiology and Biophysics, University of Tennessee,Memphis 38163J. M Krueger - Department of Physiology and Biophysics, University of Tennessee,Memphis 38163
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.263(5), pp.R1078-R1085
- Academic Unit
- Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547582401842
- Language
- English
- Resource Type
- Journal article