Journal article
Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host
Parasitology research (1987), Vol.112(9), pp.3075-3090
09/2013
Handle:
https://hdl.handle.net/2376/102989
PMID: 23749091
Abstract
As it feeds upon cattle, Rhipicephalus (Boophilus) microplus is capable of transmitting a number of pathogenic organisms, including the apicomplexan hemoparasite Babesia bovis, a causative agent of bovine babesiosis. The R. microplus female gut transcriptome was studied for two cohorts: adult females feeding on a bovine host infected with B. bovis and adult females feeding on an uninfected bovine. RNA was purified and used to generate a subtracted cDNA library from B. bovis-infected female gut, and 4,077 expressed sequence tags (ESTs) were sequenced. Gene expression was also measured by a microarray designed from the publicly available R. microplus gene index: BmiGI Version 2. We compared gene expression in the tick gut from females feeding upon an uninfected bovine to gene expression in tick gut from females feeding upon a splenectomized bovine infected with B. bovis. Thirty-three ESTs represented on the microarray were expressed at a higher level in female gut samples from the ticks feeding upon a B. bovis-infected calf compared to expression levels in female gut samples from ticks feeding on an uninfected calf. Forty-three transcripts were expressed at a lower level in the ticks feeding upon B. bovis-infected female guts compared with expression in female gut samples from ticks feeding on the uninfected calf. These array data were used as initial characterization of gene expression associated with the infection of R. microplus by B. bovis.
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Details
- Title
- Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host
- Creators
- Andrew Heekin - Knipling-Bushland US Livestock Insects Research Laboratory USDA-ARS 2700 Fredericksburg Road Kerrville TX 78028 USAFelix Guerrero - Knipling-Bushland US Livestock Insects Research Laboratory USDA-ARS 2700 Fredericksburg Road Kerrville TX 78028 USAKylie Bendele - Knipling-Bushland US Livestock Insects Research Laboratory USDA-ARS 2700 Fredericksburg Road Kerrville TX 78028 USALeo Saldivar - Department of Mathematics University of Texas at El Paso El Paso TX 79968 USAGlen Scoles - Animal Disease Research Unit USDA-ARS Pullman WA 99164 USAScot Dowd - Molecular Research 503 Clovis Road Shallowater TX 79363 USACedric Gondro - The Institute for Genetics and Bioinformatics University of New England Armidale NSW 2351 AustraliaVishvanath Nene - International Livestock Research Institute (ILRI) and Biosciences eastern and central Africa (BecA) Hub P.O. Box 30709 Nairobi KenyaAppolinaire Djikeng - International Livestock Research Institute (ILRI) and Biosciences eastern and central Africa (BecA) Hub P.O. Box 30709 Nairobi KenyaKelly Brayton - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164 USA
- Publication Details
- Parasitology research (1987), Vol.112(9), pp.3075-3090
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Springer Berlin Heidelberg; Berlin/Heidelberg
- Identifiers
- 99900546797401842
- Language
- English
- Resource Type
- Journal article