Haemonchus contortus GA1 antigens: related, phospholipase C-sensitive, apical gut membrane proteins encoded as a polyprotein and released from the nematode during infection

D P Jasmer; L E Perryman; T C McGuire

doi:10.1073/pnas.93.16.8642

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Haemonchus contortus GA1 antigens: related, phospholipase C-sensitive, apical gut membrane proteins encoded as a polyprotein and released from the nematode during infection

Journal article

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Haemonchus contortus GA1 antigens: related, phospholipase C-sensitive, apical gut membrane proteins encoded as a polyprotein and released from the nematode during infection

D P Jasmer, L E Perryman and T C McGuire

Proceedings of the National Academy of Sciences - PNAS, Vol.93(16), pp.8642-8647

08/06/1996

DOI: https://doi.org/10.1073/pnas.93.16.8642

Handle:

https://hdl.handle.net/2376/110718

PMCID: PMC38726

PMID: 8710924

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Abstract

Amino Acid Sequence

Gene Expression

Molecular Weight

Membrane Proteins - genetics

Protein Precursors - chemistry

RNA, Messenger - genetics

Molecular Sequence Data

Type C Phospholipases - metabolism

Membrane Proteins - immunology

Protein Precursors - immunology

Antigens, Helminth - genetics

Helminth Proteins - genetics

Intestines - immunology

Membrane Glycoproteins - genetics

Sequence Homology, Amino Acid

Haemonchus - genetics

Animals

Intestinal Mucosa - immunology

Base Sequence

Helminth Proteins - immunology

Membrane Glycoproteins - immunology

Intestinal Mucosa - parasitology

Sheep - immunology

It was previously shown that the Haemonchus contortus apical gut surface proteins p46, p52, and p100 induced protective immunity to challenge infections in goats. Here, it is shown that the three proteins are all encoded by a single gene (GA1) and initially expressed in adult parasites as a polyprotein (p100GA1). p46GA1 and p52GA1 are related proteins with 47% sequence identity, including a cysteine-containing region, which appears to confer secondary structure to these proteins, and a region with sequence similarity to bacterial Tolb proteins. GA1 protein expression is regulated during the life cycle at the level of transcript abundance. Only p52GA1 has characteristics of a glycosylinositolphospholipid membrane-anchored protein. However, both p46GA1 and p52GA1 were released from the gut membrane by phosphatidylinositol specific-phospholipase C, suggesting that p46GA1 membrane association depends on interactions with a glycosylinositolphospholipid gut membrane protein. Finally, GA1 proteins occur in abomasal mucus of infected lambs, demonstrating possible presentation to the host immune system during H. contortus infection. The results identify multiple characteristics of the GA1 proteins that should be considered for design of recombinant antigens for vaccine trials and that implicate a series of cellular processes leading to modification and expression of GA1 proteins at the nematode apical gut surface.

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Title: Haemonchus contortus GA1 antigens: related, phospholipase C-sensitive, apical gut membrane proteins encoded as a polyprotein and released from the nematode during infection
Creators: D P Jasmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA
L E Perryman
T C McGuire
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.93(16), pp.8642-8647
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Identifiers: 99900547175501842
Language: English
Resource Type: Journal article