Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice

Levinus A Dieleman; Annemarie Arends; Susan L Tonkonogy; Marije S Goerres; David W Craft; Wetonia Grenther; Rance K Sellon; Ed Balish; R. Balfour Sartor

doi:10.1128/IAI.68.9.5107-5113.2000

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Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice

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Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice

Levinus A Dieleman, Annemarie Arends, Susan L Tonkonogy, Marije S Goerres, David W Craft, Wetonia Grenther, Rance K Sellon, Ed Balish and R. Balfour Sartor

Infection and immunity, Vol.68(9), pp.5107-5113

09/2000

DOI: https://doi.org/10.1128/IAI.68.9.5107-5113.2000

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https://hdl.handle.net/2376/109284

PMCID: PMC101749

PMID: 10948132

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https://doi.org/10.1128/IAI.68.9.5107-5113.2000View

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Abstract

Host Response and Inflammation

Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10 −/− mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10 −/− mice. Germfree IL-10 −/− mice on either a mixed (C57BL/6 × 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10 −/− mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus . After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10 −/− mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus . We conclude that H. hepaticus does not induce or potentiate disease in our IL-10 −/− mice and therefore is not required to induce colitis in genetically susceptible hosts.

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Title: Helicobacter hepaticus Does Not Induce or Potentiate Colitis in Interleukin-10-Deficient Mice
Creators: Levinus A Dieleman - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Annemarie Arends - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Susan L Tonkonogy - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Marije S Goerres - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
David W Craft - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Wetonia Grenther - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Rance K Sellon - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Ed Balish - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
R. Balfour Sartor - Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill
Publication Details: Infection and immunity, Vol.68(9), pp.5107-5113
Academic Unit: Veterinary Clinical Sciences, Department of
Publisher: American Society for Microbiology
Identifiers: 99900547010301842
Language: English
Resource Type: Journal article