Journal article
Heterologous strain challenge of cattle immunized with Anaplasma marginale outer membranes
Veterinary immunology and immunopathology, Vol.42(3-4), pp.265-273
09/1994
Handle:
https://hdl.handle.net/2376/105677
PMID: 7810060
Abstract
In this study, we tested the hypothesis that outer membrane immunization would induce protection against an antigenically variant strain. Outer membranes were isolated from the Virginia strain of Anaplasma marginale using density gradient centrifugation, combined with saponin adjuvant, and used to immunize Friesian cattle in Zimbabwe. Immunized cattle developed high antibody titers (80 000–160 000) against outer membrane polypeptides including MSP-2 and MSP-5 in both the homologous Virginia and heterologous Zimbabwe strains. Outer membrane immunized cattle were protected significantly following challenge with 104 Zimbabwe strain parasitized erythrocytes, demonstrated by significant differences in prepatent period and peak rickettsemia compared with adjuvant immunized control cattle. One outer membrane immunized animal was completely protected against infection. However, there were no overall significant differences in severity of anemia between cattle immunized with outer membrane and the control group, indicating that a significant reduction in rickettsemia does not necessarily result in less severe anemia.
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Details
- Title
- Heterologous strain challenge of cattle immunized with Anaplasma marginale outer membranes
- Creators
- Guy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USADevere Munodzana - Veterinary Research Laboratory, P.O. Box 8101, Causeway, Harare, ZimbabweNtando Tebele - Veterinary Research Laboratory, P.O. Box 8101, Causeway, Harare, ZimbabweTendai Ushe - Veterinary Research Laboratory, P.O. Box 8101, Causeway, Harare, ZimbabweTerry F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.42(3-4), pp.265-273
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900546968301842
- Language
- English
- Resource Type
- Journal article