Journal article
High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety
PloS one, Vol.10(10), pp.e0139775-e0139775
2015
Handle:
https://hdl.handle.net/2376/105773
PMCID: PMC4595016
PMID: 26440522
Abstract
Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. fulvum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 × G0005527 F2 population.
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Details
- Title
- High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety
- Creators
- Tao Yang - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaLi Fang - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaXiaoyan Zhang - Qingdao Academy of Agricultural Sciences, Qingdao, ChinaJinguo Hu - USDA-ARS Western Regional Plant Introduction Station, Pullman, Washington, United States of AmericaShiying Bao - Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming, ChinaJunjie Hao - Qingdao Academy of Agricultural Sciences, Qingdao, ChinaLing Li - Institute of Cash Crops, Liaoning Academy of Agricultural Sciences, Liaoyang, ChinaYuhua He - Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming, ChinaJunye Jiang - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaFang Wang - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaShufang Tian - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaXuxiao Zong - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
- Publication Details
- PloS one, Vol.10(10), pp.e0139775-e0139775
- Academic Unit
- Agricultural, Human, and Natural Resource Sciences, College of
- Publisher
- United States
- Identifiers
- 99900546728901842
- Language
- English
- Resource Type
- Journal article