Journal article
High consistency enzymatic hydrolysis of hardwood substrates
Bioresource technology, Vol.100(23), pp.5890-5897
2009
Handle:
https://hdl.handle.net/2376/114169
PMID: 19643602
Abstract
The feasibility of using a laboratory peg mixer to carry out high consistency enzymatic hydrolysis of lignocellulosic substrates was investigated. Two hardwood substrates, unbleached hardwood pulp (UBHW) and organosolv pretreated poplar (OPP), were used in this study. Hydrolysis of UBHW and OPP at 20% substrate consistency led to a high glucose concentration in the final hydrolysate. For example, a 48
h enzymatic hydrolysis of OPP resulted in a hydrolysate with 158
g/L of glucose. This is the highest glucose concentration ever obtained from enzymatic hydrolysis of lignocellulosic substrates. Fermentation of UBHW and OPP hydrolysates with high glucose content led to high ethanol concentrations, 50.4 and 63.1
g/L, respectively after fermentation. Our results demonstrate that using common pulping equipment to carry out high consistency hydrolysis can overcome the rheological problems and greatly increase the sugar and ethanol concentrations after the hydrolysis and fermentation.
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Details
- Title
- High consistency enzymatic hydrolysis of hardwood substrates
- Creators
- Xiao Zhang - FPInnovations Paprican Division (PAPRICAN), 570 boul. St-Jean, Pointe-Claire, Quebec, Canada H9R 3J9Wenjuan Qin - Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancovuer, BC, Canada V6T 1Z4Michael G Paice - FPInnovations Paprican Division (PAPRICAN), 570 boul. St-Jean, Pointe-Claire, Quebec, Canada H9R 3J9John N Saddler - Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancovuer, BC, Canada V6T 1Z4
- Publication Details
- Bioresource technology, Vol.100(23), pp.5890-5897
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547592701842
- Language
- English
- Resource Type
- Journal article