Journal article
High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus
Nature communications, Vol.4(1), pp.2673-2673
10/23/2013
Handle:
https://hdl.handle.net/2376/113002
PMCID: PMC3826619
PMID: 24150273
Abstract
Stripe rust, caused by
Puccinia striiformis
f. sp.
tritici
(
Pst
), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of
Pst
isolate CY32, obtained using a ‘fosmid-to-fosmid’ strategy, to better understand its race evolution and pathogenesis. The
Pst
genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens,
Pst
has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in
Pst
suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the ‘fosmid-to-fosmid’ strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in
Pst
and other obligate pathogens.
Stripe rust is one of the most destructive wheat diseases. Here, Zheng and colleagues report a draft genome sequence of wheat stripe rust fungus, generated using a fosmid-to-fosmid approach, and provide insight into its race evolution and pathogenesis.
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Details
- Title
- High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus
- Creators
- Wenming Zheng - , Yangling, Shaanxi 712100Lili Huang - , Yangling, Shaanxi 712100Jinqun Huang - , Shenzhen 518083Xiaojie Wang - , Yangling, Shaanxi 712100Xianming Chen - , Pullman, Washington 99164-6430Jie Zhao - , Yangling, Shaanxi 712100Jun Guo - , Yangling, Shaanxi 712100Hua Zhuang - , Yangling, Shaanxi 712100Chuangzhao Qiu - , Shenzhen 518083Jie Liu - , Yangling, Shaanxi 712100Huiquan Liu - , Yangling, Shaanxi 712100Xueling Huang - , Yangling, Shaanxi 712100Guoliang Pei - , Yangling, Shaanxi 712100Gangming Zhan - , Yangling, Shaanxi 712100Chunlei Tang - , Yangling, Shaanxi 712100Yulin Cheng - , Yangling, Shaanxi 712100Minjie Liu - , Yangling, Shaanxi 712100Jinshan Zhang - , Yangling, Shaanxi 712100Zhongtao Zhao - , Yangling, Shaanxi 712100Shijie Zhang - , Yangling, Shaanxi 712100Qingmei Han - , Yangling, Shaanxi 712100Dejun Han - , Yangling, Shaanxi 712100Hongchang Zhang - , Yangling, Shaanxi 712100Jing Zhao - , Yangling, Shaanxi 712100Xiaoning Gao - , Yangling, Shaanxi 712100Jianfeng Wang - , Yangling, Shaanxi 712100Peixiang Ni - , Shenzhen 518083Wei Dong - , Shenzhen 518083Linfeng Yang - , Shenzhen 518083Huanming Yang - , Shenzhen 518083Jin-Rong Xu - , West Lafayette, IN 47907Gengyun Zhang - , Shenzhen 518083Zhensheng Kang - , Yangling, Shaanxi 712100
- Publication Details
- Nature communications, Vol.4(1), pp.2673-2673
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Nature Pub. Group
- Identifiers
- 99900547659001842
- Language
- English
- Resource Type
- Journal article