Journal article
High-oleate peanut mutants result from a MITE insertion into the FAD2 gene
Theoretical and applied genetics, Vol.108(8), pp.1492-1502
05/2004
Handle:
https://hdl.handle.net/2376/103918
PMID: 14968307
Abstract
A high-oleate trait in the cultivated peanut (Arachis hypogaea L.) was reported to rely on the allelic composition of the two homeologous, microsomal oleoyl-PC desaturase genes (ahFAD2A or ahFAD2B). The enzyme activity of either ahFAD2A or ahFAD2B is sufficient for a normal oleate phenotype, and a significant reduction in the levels of ahFAD2B and a mutation in ahFAD2A were reported to be responsible for the high-oleate phenotype in one chemically induced mutant (M2-225) and one derived from a naturally occurring (8-2122) mutant. Here, we report an insertion of the same miniature inverted-repeat transposable element (MITE) in the ahFAD2B gene in another chemically induced mutant (Mycogen-Flavo) and the previously characterized M2-225 mutant. In both cases, this MITE insertion in ahFAD2B causes a frameshift, resulting in a putatively truncated protein sequence in both mutants. The insertion of this MITE in ahFAD2B, in addition to the point mutation in ahFAD2A, appears to be the cause of the high-oleate phenotype in Mycogen-Flavo and M2-225 mutants. Utilizing sequences of the MITE, we developed a DNA marker strategy to differentiate the two insertion-containing mutants from the normal oleate peanut variety (AT-108) and the naturally occurring, high-oleate mutant 8-2122. Reverse transcript-PCR/differential digestion results reveal the expression of both ahFAD2A and ahFAD2B genes in Mycogen-Flavo mutant. This result is in contrast to the observation that ahFAD2B transcripts are greatly reduced in the M2-225 mutant having the MITE insertion further 3′ in ahFAD2B gene.
Metrics
10 Record Views
Details
- Title
- High-oleate peanut mutants result from a MITE insertion into the FAD2 gene
- Creators
- M Patel - Department of Genetics and Biochemistry Clemson University 122 Long Hall Clemson SC 29634-0324 USAS Jung - Department of Genetics and Biochemistry Clemson University 122 Long Hall Clemson SC 29634-0324 USAK Moore - AgResearch Consultants Inc. Ashburn GA 31714 USAG Powell - Department of Genetics and Biochemistry Clemson University 122 Long Hall Clemson SC 29634-0324 USAC Ainsworth - Plant Molecular Biology Laboratory Imperial College London Wye Campus, Wye Ashford Kent TN25 5AH UKA Abbott - Department of Genetics and Biochemistry Clemson University 122 Long Hall Clemson SC 29634-0324 USA
- Publication Details
- Theoretical and applied genetics, Vol.108(8), pp.1492-1502
- Academic Unit
- Agricultural, Human, and Natural Resource Sciences, College of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900546655101842
- Language
- English
- Resource Type
- Journal article