Journal article
High-performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats
Biomedical chromatography, Vol.24(5), pp.516-521
05/2010
Handle:
https://hdl.handle.net/2376/115450
PMID: 19739255
Abstract
A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5-100.0 microg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069-18.4% (RSD%), and within 20% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis.
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Details
- Title
- High-performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats
- Creators
- Rebecca M Bertram - Department of Pharmaceutical Sciences and Pharmacology, Washington State University, Pullman, 99164-6534, USAJody K TakemotoConnie M RemsbergKarina R Vega-VillaShyam SablaniNeal M Davies
- Publication Details
- Biomedical chromatography, Vol.24(5), pp.516-521
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- England
- Identifiers
- 99900547602701842
- Language
- English
- Resource Type
- Journal article