Journal article
High resolution imaging to assess oilseed species’ root hair responses to soil water stress
Plant and soil, Vol.339(1), pp.125-135
02/2011
Handle:
https://hdl.handle.net/2376/114079
Abstract
An imaging method was developed to evaluate crop species differences in root hair morphology using high resolution scanners, and to determine if the method could also detect root hair responses to soil water availability. High resolution (1890 picture elements (pixels) cm−1) desktop scanners were buried in containers filled with soil to characterize root hair development under two water availability levels (−63 and −188 kPa) for canola (Brassica napus L. cv Clearwater), camelina (Camelina sativa L. Crantz cv Cheyenne), flax (Linum usitatissimum L. cv CDC Bethune), and lentil (Lens culinaris Medik. cv Brewer). There was notable effect of available moisture on root hair geometry (RHG). At −188 kPa, length from the root tip to the root hair initiation zone decreased and root hair length (RHL) became more variable near the root hair initiation zone as compared to −63 kPa. For the response of primary axial RHL, significant main effects were present for both water availability (P < 0.05) and species (P < 0.0001); lateral RHL showed a significant main effect for both water availability (P < 0.05) and species (P < 0.01) as well. For both primary axial and lateral root hair density (RHD), there was a significant effect of species (P < 0.0001), but no significant response to water availability. No water availability x species interaction was present in any case. Low available water reduced RHL in both primary axial and lateral roots. The change in RHL due to water availability was most evident in canola and camelina. Additionally, those with greater RHL
$$ \left( {\text{canola} = \text{camelina} > \text{flax} = \text{lentil}} \right) $$
had lower RHD
$$ \left( {\text{canola} = \text{camelina} < \text{flax} < \text{lentil}} \right) $$
in primary axial roots and a similar trend was found in lateral RHL. Both water and species had a significant effect on primary axial root surface area (RSA) (P < 0.05) but no significant effect was found for lateral RSA. For primary axial RSA the longest and most dense root hair had the greatest RSA. This novel approach to in situ rhizosphere imaging allowed observation of species differences in root hair development in response to water availability and should be useful in future studies of rhizosphere interactions and crop water and nutrient management.
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Details
- Title
- High resolution imaging to assess oilseed species’ root hair responses to soil water stress
- Creators
- W Hammac - Department of Crop and Soil Sciences Washington State University P.O. Box 646420 Pullman WA 99164-6420 USAWilliam Pan - Department of Crop and Soil Sciences Washington State University P.O. Box 646420 Pullman WA 99164-6420 USARon Bolton - Department of Crop and Soil Sciences Washington State University P.O. Box 646420 Pullman WA 99164-6420 USARich Koenig - Department of Crop and Soil Sciences Washington State University P.O. Box 646420 Pullman WA 99164-6420 USA
- Contributors
- Johan Six (Editor)
- Publication Details
- Plant and soil, Vol.339(1), pp.125-135
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547916201842
- Language
- English
- Resource Type
- Journal article