Journal article
High-throughput sequencing and pathway analysis reveal alteration of the pituitary transcriptome by 17α-ethynylestradiol (EE2) in female coho salmon, Oncorhynchus kisutch
Aquatic toxicology, Vol.142-143, pp.146-163
10/15/2013
Handle:
https://hdl.handle.net/2376/105714
PMID: 24007788
Abstract
•Studied impacts of ethynylestradiol (EE2) exposure on salmon pituitary transcriptome.•High-throughput sequencing, RNAseq, and pathway analysis were performed.•EE2 altered mRNAs for genes in circadian rhythm, GnRH, and TGFβ signaling pathways.•LH and FSH beta subunit mRNAs were most highly up- and down-regulated by EE2, respectively.•Estrogens may alter processes associated with reproductive timing in salmon.
Considerable research has been done on the effects of endocrine disrupting chemicals (EDCs) on reproduction and gene expression in the brain, liver and gonads of teleost fish, but information on impacts to the pituitary gland are still limited despite its central role in regulating reproduction. The aim of this study was to further our understanding of the potential effects of natural and synthetic estrogens on the brain–pituitary–gonad axis in fish by determining the effects of 17α-ethynylestradiol (EE2) on the pituitary transcriptome. We exposed sub-adult coho salmon (Oncorhynchus kisutch) to 0 or 12ng EE2/L for up to 6 weeks and effects on the pituitary transcriptome of females were assessed using high-throughput Illumina® sequencing, RNA-Seq and pathway analysis. After 1 or 6 weeks, 218 and 670 contiguous sequences (contigs) respectively, were differentially expressed in pituitaries of EE2-exposed fish relative to control. Two of the most highly up- and down-regulated contigs were luteinizing hormone β subunit (241-fold and 395-fold at 1 and 6 weeks, respectively) and follicle-stimulating hormone β subunit (−3.4-fold at 6 weeks). Additional contigs related to gonadotropin synthesis and release were differentially expressed in EE2-exposed fish relative to controls. These included contigs involved in gonadotropin releasing hormone (GNRH) and transforming growth factor-β signaling. There was an over-representation of significantly affected contigs in 33 and 18 canonical pathways at 1 and 6 weeks, respectively, including circadian rhythm signaling, calcium signaling, peroxisome proliferator-activated receptor (PPAR) signaling, PPARα/retinoid x receptor α activation, and netrin signaling. Network analysis identified potential interactions between genes involved in circadian rhythm and GNRH signaling, suggesting possible effects of EE2 on timing of reproductive events.
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Details
- Title
- High-throughput sequencing and pathway analysis reveal alteration of the pituitary transcriptome by 17α-ethynylestradiol (EE2) in female coho salmon, Oncorhynchus kisutch
- Creators
- Louisa B Harding - School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USAIrvin R Schultz - Battelle, Marine Sciences Laboratory – Pacific Northwest National Laboratory, 1529 West Sequim Bay Road, Sequim, WA 98382, USAGiles W Goetz - School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USAJ. Adam Luckenbach - Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 2725 Montlake Blvd E, Seattle, WA 98112, USAGraham Young - School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USAFrederick W Goetz - Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Manchester Research Station, P.O. Box 130, Manchester, WA 98353, USAPenny Swanson - Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 2725 Montlake Blvd E, Seattle, WA 98112, USA
- Publication Details
- Aquatic toxicology, Vol.142-143, pp.146-163
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier B.V
- Identifiers
- 99900546929001842
- Language
- English
- Resource Type
- Journal article