Journal article
Highly stable enzyme precipitate coatings and their electrochemical applications
Biosensors & bioelectronics, Vol.26(5), pp.1980-1986
2011
Handle:
https://hdl.handle.net/2376/104482
PMID: 20951568
Abstract
This paper describes highly stable enzyme precipitate coatings (EPCs) on electrospun polymer nanofibers and carbon nanotubes (CNTs), and their potential applications in the development of highly sensitive biosensors and high-powered biofuel cells. EPCs of glucose oxidase (GOx) were prepared by precipitating GOx molecules in the presence of ammonium sulfate, then cross-linking the precipitated GOx aggregates on covalently attached enzyme molecules on the surface of nanomaterials. EPCs-GOx not only improved enzyme loading, but also retained high enzyme stability. For example, EPC-GOx on CNTs showed a 50 times higher activity per unit weight of CNTs than the conventional approach of covalent attachment, and its initial activity was maintained with negligible loss for 200 days. EPC-GOx on CNTs was entrapped by Nafion to prepare enzyme electrodes for glucose sensors and biofuel cells. The EPC-GOx electrode showed a higher sensitivity and a lower detection limit than an electrode prepared with covalently attached GOx (CA-GOx). The CA-GOx electrode showed an 80% drop in sensitivity after thermal treatment at 50
°C for 4
h, while the EPC-GOx electrode maintained its high sensitivity with negligible decrease under the same conditions. The use of EPC-GOx as the anode of a biofuel cell improved the power density, which was also stable even after thermal treatment of the enzyme anode at 50
°C. The excellent stability of the EPC-GOx electrode together with its high current output create new potential for the practical applications of enzyme-based glucose sensors and biofuel cells.
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Details
- Title
- Highly stable enzyme precipitate coatings and their electrochemical applications
- Creators
- Byoung Chan Kim - Environment Division, Korea Institute of Science and Technology, Seoul 136-791, Republic of KoreaXueyan Zhao - Department of Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USAHye-Kyung Ahn - Department of Chemical Engineering, Chungbuk National University, Chungbuk, Cheongju 361-763, Republic of KoreaJae Hyun Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of KoreaHye-Jin Lee - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of KoreaKyung Woo Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of KoreaSujith Nair - Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802, USAErik Hsiao - Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802, USAHongfei Jia - Department of Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USAMin-Kyu Oh - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of KoreaByoung In Sang - Hazardous Substances Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of KoreaBeom-Soo Kim - Department of Chemical Engineering, Chungbuk National University, Chungbuk, Cheongju 361-763, Republic of KoreaSeong H Kim - Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802, USAYongchai Kwon - Department of Chemical and Environmental Technology, Inha Technical College, Incheon 402-752, Republic of KoreaSu Ha - Department of Chemical Engineering, Washington State University, Pullman, WA 99164, USAMan Bock Gu - College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of KoreaPing Wang - Department of Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USAJungbae Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea
- Publication Details
- Biosensors & bioelectronics, Vol.26(5), pp.1980-1986
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546970301842
- Language
- English
- Resource Type
- Journal article