Journal article
Host-Induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat
Methods in molecular biology (Clifton, N.J.), Vol.1848, pp.139-150
2018
Handle:
https://hdl.handle.net/2376/115811
PMID: 30182235
Abstract
Biotrophic fungi (Puccinia spp.) cause devastating diseases of wheat and other cereal species globally. The function of large repertories of genes from Puccinia spp. still needs to be discovered to understand the infection process of these obligate parasites, eventually to protect plants from rust diseases. Functional analysis of targeted genes is challenging due to the inherent difficulties with culturing the fungus and transforming the host. RNA interference (RNAi) is a conserved gene regulation process in eukaryotes and known to be a powerful genetic tool in plant biotechnology. More recently, host-induced gene silencing (HIGS) has been developed to assess pathogen gene function in plants. HIGS is an RNAi-based process where double stranded RNA (dsRNA) homologous to a pathogen gene can be expressed in a plant to induce targeted silencing of the pathogen gene. Here we described a detailed HIGS protocol for functional analysis of rust genes from Puccinia species in cereals. As an example we describe an experiment silencing the tryptophan 2-monooxygenase gene (Pgt-IaaM) from Puccinia graminis f. sp. tritici (Pgt) that is involved in virulence to wheat.
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Details
- Title
- Host-Induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat
- Creators
- Chuntao Yin - Department of Plant Pathology, Washington State University, Pullman, WA, USAScot H Hulbert - Department of Plant Pathology, Washington State University, Pullman, WA, USA. scot_hulbert@wsu.edu
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1848, pp.139-150
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900548155801842
- Language
- English
- Resource Type
- Journal article