Host plant resistance against tomato spotted wilt virus in peanut (Arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival

Sivamani Sundaraj; Rajagopalbabu Srinivasan; Albert K Culbreath; David G Riley; Hanu R Pappu

doi:10.1094/PHYTO-04-13-0107-R

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Host plant resistance against tomato spotted wilt virus in peanut (Arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival

Journal article

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Host plant resistance against tomato spotted wilt virus in peanut (Arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival

Sivamani Sundaraj, Rajagopalbabu Srinivasan, Albert K Culbreath, David G Riley and Hanu R Pappu

Phytopathology, Vol.104(2), pp.202-210

02/2014

DOI: https://doi.org/10.1094/PHYTO-04-13-0107-R

Handle:

https://hdl.handle.net/2376/101989

PMID: 24025049

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Abstract

Haplotypes

Genetics, Population

Tospovirus - genetics

Plant Diseases - immunology

Plant Leaves

Arachis - genetics

Thysanoptera - physiology

Arachis - virology

Feeding Behavior

Genotype

Phylogeny

Insect Vectors - virology

Seedlings

Host-Pathogen Interactions

Animals

Insect Vectors - physiology

Nucleocapsid Proteins - genetics

Plant Diseases - virology

Arachis - immunology

Arachis - parasitology

Georgia

Mutation

Thysanoptera - virology

Tospovirus - physiology

Tomato spotted wilt virus (TSWV) severely affects peanut production in the southeastern United States. Breeding efforts over the last three decades resulted in the release of numerous peanut genotypes with field resistance to TSWV. The degree of field resistance in these genotypes has steadily increased over time, with recently released genotypes exhibiting a higher degree of field resistance than older genotypes. However, most new genotypes have never been evaluated in the greenhouse or laboratory against TSWV or thrips, and the mechanism of resistance is unknown. In this study, TSWV-resistant and -susceptible genotypes were subjected to TSWV mechanical inoculation. The incidence of TSWV infection was 71.7 to 87.2%. Estimation of TSWV nucleocapsid (N) gene copies did not reveal significant differences between resistant and susceptible genotypes. Parsimony and principal component analyses of N gene nucleotide sequences revealed inconsistent differences between virus isolates collected from resistant and susceptible genotypes and between old (collected in 1998) and new (2010) isolates. Amino acid sequence analyses indicated consistent differences between old and new isolates. In addition, we found evidence for overabundance of nonsynonymous substitutions. However, there was no evidence for positive selection. Purifying selection, population expansion, and differentiation seem to have influenced the TSWV populations temporally rather than positive selection induced by host resistance. Choice and no-choice tests indicated that resistant and susceptible genotypes differentially affected thrips feeding and survival. Thrips feeding and survival were suppressed on some resistant genotypes compared with susceptible genotypes. These findings reveal how TSWV resistance in peanut could influence evolution, epidemiology, and management of TSWV.

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Title: Host plant resistance against tomato spotted wilt virus in peanut (Arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival
Creators: Sivamani Sundaraj
Rajagopalbabu Srinivasan
Albert K Culbreath
David G Riley
Hanu R Pappu
Publication Details: Phytopathology, Vol.104(2), pp.202-210
Academic Unit: Plant Pathology, Department of
Publisher: United States
Identifiers: 99900546509901842
Language: English
Resource Type: Journal article