Hsp70 positively regulates porcine circovirus type 2 replication in vitro

Jie Liu; Juan Bai; Lili Zhang; Zhihua Jiang; Xianwei Wang; Yufeng Li; Ping Jiang

doi:10.1016/j.virol.2013.08.025

Back

Hsp70 positively regulates porcine circovirus type 2 replication in vitro

Journal article

Open access

Peer reviewed

Hsp70 positively regulates porcine circovirus type 2 replication in vitro

Jie Liu, Juan Bai, Lili Zhang, Zhihua Jiang, Xianwei Wang, Yufeng Li and Ping Jiang

Virology (New York, N.Y.), Vol.447(1-2), pp.52-62

12/2013

DOI: https://doi.org/10.1016/j.virol.2013.08.025

Handle:

https://hdl.handle.net/2376/101123

PMID: 24210099

Files and links (1)

url

https://doi.org/10.1016/j.virol.2013.08.025View

Published (Version of record) Open

Abstract

Heat shock response

NF-κB

Porcine circovirus type 2

Hsp70

Caspase-3

The Hsp70 chaperone plays a central role in multiple processes within cells. Porcine circovirus type 2 (PCV2) is the essential causal agent of post-weaning multisystemic wasting syndrome (PMWS), which has spread worldwide. But the mechanism of PCV2 replication remains poorly understood. In this study, we firstly found the positive effect of heat stress on the replication of PCV2 in the continuous porcine monocytic cell line 3D4/31. Downregulation of Hsp70 by the specific chaperone inhibitor Quercetin or RNA interference and upregulation of Hsp70 by expression from a recombinant adenovirus showed that Hsp70 enhanced PCV2 genome replication and virion production. A specific interaction between Hsp70 and PCV2 Cap was confirmed by colocalization by confocal microscopy and co-immunoprecipitation. Furthermore, the NF-κB pathway was activated and caspase-3 activity was reduced when Hsp70 was overexpressed in PCV2-infected 3D4/31 cells. These data suggested that Hsp70 positively regulated PCV2 replication, which being helpful for understanding the molecular mechanism of PCV2 infection. [Display omitted] •Heat shock could increase PCV2 replication and progeny production in 3D4/31 cell line.•Hsp70 had a positive regulatory effect on the PCV2 infection cycle.•Hsp70 could interact with PCV2 Cap protein in during the viral replication.•Hsp70 increased PCV2 NF-κB pathway activation and blocked PCV2 caspase-3 activity.

Metrics

16 Record Views

Details

Title: Hsp70 positively regulates porcine circovirus type 2 replication in vitro
Creators: Jie Liu - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Juan Bai - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Lili Zhang - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Zhihua Jiang - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA
Xianwei Wang - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Yufeng Li - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Ping Jiang - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Publication Details: Virology (New York, N.Y.), Vol.447(1-2), pp.52-62
Academic Unit: Animal Sciences, Department of; Languages, Cultures, and Race, School of
Publisher: Elsevier Inc
Identifiers: 99900546555201842
Language: English
Resource Type: Journal article