Journal article
Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia
Microbes and infection, Vol.17(4), pp.266-274
04/2015
Handle:
https://hdl.handle.net/2376/104670
PMID: 25576024
Abstract
Phylogenetic group D extraintestinal pathogenic Escherichia coli (ExPEC), including O15:K52:H1 and clonal group A, have spread globally and become fluoroquinolone-resistant. Here we investigated the role of canine feces as a reservoir of these (and other) human-associated ExPEC and their potential as canine pathogens. We characterized and compared fluoroquinolone-resistant E. coli isolates originally identified as phylogenetic group D from either the feces of hospitalized dogs (n = 67; 14 dogs) or extraintestinal infections (n = 53; 33 dogs). Isolates underwent phylogenetic grouping, random amplified polymorphic DNA (RAPD) analysis, virulence genotyping, resistance genotyping, human-associated ExPEC O-typing, and multi-locus sequence typing. Five of seven human-associated sequence types (STs) exhibited ExPEC-associated O-types, and appeared in separate RAPD clusters. The largest subgroup (16 fecal, 26 clinical isolates) were ST354 (phylogroup F) isolates. ST420 (phylogroup B2); O1-ST38, O15:K52:H1-ST393, and O15:K1-ST130 (phylogroup D); and O7-ST457, and O1-ST648 (phylogroup F) were also identified. Three ST-specific RAPD sub-clusters (ST354, ST393, and ST457) contained closely related isolates from both fecal or clinical sources. Genes encoding CTX-M and AmpC β-lactamases were identified in isolates from five STs. Major human-associated fluoroquinolone-resistant ± extended-spectrum cephalosporin-resistant ExPEC of public health importance may be carried in dog feces and cause extraintestinal infections in some dogs.
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Details
- Title
- Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia
- Creators
- SiYu Guo - School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaDavid Wakeham - School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5371, AustraliaHuub J.M Brouwers - Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Woodbridge Road, NSW 2568, AustraliaRowland N Cobbold - School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaSam Abraham - School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5371, AustraliaJoanne L Mollinger - School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaJames R Johnson - Infectious Diseases (111F), VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USAToni A Chapman - Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Woodbridge Road, NSW 2568, AustraliaDavid M Gordon - School of Biology, The Australian National University, Acton, ACT 0200, AustraliaVanessa R Barrs - University Veterinary Teaching Hospital, Sydney, Faculty of Veterinary Science, The University of Sydney, NSW 2006, AustraliaDarren J Trott - School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5371, Australia
- Publication Details
- Microbes and infection, Vol.17(4), pp.266-274
- Publisher
- Elsevier Masson SAS
- Identifiers
- 99900547086701842
- Language
- English
- Resource Type
- Journal article