Journal article
Human norovirus infection of Caco-2 cells grown as a three-dimensional tissue structure
Journal of water and health, Vol.9(2), pp.225-240
06/01/2011
Handle:
https://hdl.handle.net/2376/109842
PMID: 21942189
Abstract
Human norovirus (hNoV) infectivity was studied using a three-dimensional model of large intestinal epithelium. Large intestine Caco-2 cells were grown in rotating wall vessel bioreactors for 18–21 days at 37°C and then transferred to 24-well tissue culture plates where they were infected with GI.1 and GII.4 human noroviruses collected from human challenge trials and various outbreak settings, respectively. Compared with uninfected cells, transmission micrographs of norovirus-infected cells displayed evidence of shortening or total loss of apical microvilli, and vacuolization. Quantitative reverse transcription real-time PCR (qRT-PCR) indicated an approximate 2–3 log10 increase in viral RNA copies for the infected cells. A passage experiment examined both the ability for continued viral RNA and viral antigen detection. In the passaged samples 1.01 × 106 copies ml−1 were detected by qRT-PCR. Immune electron microscopy using primary antibody to hNoV GI.1 capsids in conjunction with 6 nm gold-labelled secondary antibodies was performed on crude cellular lysates. Localization of antibody was observed in infected but not for uninfected cells. Our present findings, coupled with earlier work with the three-dimensional small intestinal INT407 model, demonstrate the utility of 3-D cell culture methods to develop infectivity assays for enteric viruses that do not readily infect mammalian cell cultures.
Metrics
9 Record Views
Details
- Title
- Human norovirus infection of Caco-2 cells grown as a three-dimensional tissue structure
- Creators
- Timothy M Straub - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USARachel A Bartholomew - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USACatherine O Valdez - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USANancy B Valentine - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USAAlice Dohnalkova - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USARichard M Ozanich - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USACynthia J Bruckner-Lea - Pacific Northwest National Laboratory, Chemical and Biological Signature Sciences Group, National Security Directorate, PO Box 999 MS P7-50, Richland, WA 99354, USADouglas R Call - Washington State University, Veterinary Microbiology and Pathology, 402 Bustad Hall, PO Box 647040, Pullman, WA 99164-7040, USA
- Publication Details
- Journal of water and health, Vol.9(2), pp.225-240
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Identifiers
- 99900547264501842
- Language
- English
- Resource Type
- Journal article