Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin

T Yang; B W Poovaiah

doi:10.1073/pnas.052564899

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Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin

Journal article

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Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin

T Yang and B W Poovaiah

Proceedings of the National Academy of Sciences - PNAS, Vol.99(6), pp.4097-4102

03/19/2002

DOI: https://doi.org/10.1073/pnas.052564899

Handle:

https://hdl.handle.net/2376/115793

PMCID: PMC122654

PMID: 11891305

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Abstract

Arabidopsis - enzymology

Homeostasis

Molecular Sequence Data

Arabidopsis Proteins - metabolism

Catalase - isolation & purification

Cloning, Molecular

Catalysis

Plant Leaves - enzymology

Amino Acid Sequence

Arabidopsis Proteins - genetics

Arabidopsis - drug effects

Calcium - pharmacology

Catalase - genetics

Hydrogen Peroxide - metabolism

Arabidopsis - genetics

Arabidopsis Proteins - isolation & purification

Catalase - metabolism

Sequence Alignment

Arabidopsis Proteins - chemistry

Models, Biological

Protein Binding

Enzyme Activation

Peroxisomes - enzymology

Tobacco - enzymology

Calcium-Calmodulin-Dependent Protein Kinases - metabolism

Catalase - chemistry

Environmental stimuli such as UV, pathogen attack, and gravity can induce rapid changes in hydrogen peroxide (H(2)O(2)) levels, leading to a variety of physiological responses in plants. Catalase, which is involved in the degradation of H(2)O(2) into water and oxygen, is the major H(2)O(2)-scavenging enzyme in all aerobic organisms. A close interaction exists between intracellular H(2)O(2) and cytosolic calcium in response to biotic and abiotic stresses. Studies indicate that an increase in cytosolic calcium boosts the generation of H(2)O(2). Here we report that calmodulin (CaM), a ubiquitous calcium-binding protein, binds to and activates some plant catalases in the presence of calcium, but calcium/CaM does not have any effect on bacterial, fungal, bovine, or human catalase. These results document that calcium/CaM can down-regulate H(2)O(2) levels in plants by stimulating the catalytic activity of plant catalase. Furthermore, these results provide evidence indicating that calcium has dual functions in regulating H(2)O(2) homeostasis, which in turn influences redox signaling in response to environmental signals in plants.

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Title: Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin
Creators: T Yang - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164-6414, USA
B W Poovaiah
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.99(6), pp.4097-4102
Academic Unit: Horticulture, Department of
Publisher: United States
Identifiers: 99900547820701842
Language: English
Resource Type: Journal article