Journal article
Hydrogen sulfide detection using nucleophilic substitution-cyclization-based fluorescent probes
Methods in enzymology, Vol.554, pp.47-62
2015
Handle:
https://hdl.handle.net/2376/106500
PMID: 25725515
Abstract
While previously known as a toxic pollutant, hydrogen sulfide (H2S) has been recently classified as an important signaling molecule with potent cytoprotective actions. The study on H2S is a rapidly growing field in biomedical research. Nevertheless, the detection of H2S in biological samples is still a challenge. Fluorescent assays can be very useful in this field owing to their high sensitivity and convenience. Based on the dual nucleophilicity of hydrogen sulfide, a series of reaction-based fluorescence "turn-on" probes have been developed by our lab. In this chapter, we provide an overview of the reactivity/property of these probes and detailed protocols for the use of them in experiments.
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Details
- Title
- Hydrogen sulfide detection using nucleophilic substitution-cyclization-based fluorescent probes
- Creators
- Bo Peng - Department of Chemistry, Washington State University, Pullman, Washington, USAMing Xian - Department of Chemistry, Washington State University, Pullman, Washington, USA. Electronic address: mxian@wsu.edu
- Publication Details
- Methods in enzymology, Vol.554, pp.47-62
- Academic Unit
- Chemistry, Department of
- Publisher
- United States
- Grant note
- R01HL116571 / NHLBI NIH HHS
- Identifiers
- 99900546713401842
- Language
- English
- Resource Type
- Journal article