Journal article
IFN-γ is essential for the inhibition of B16BL6 melanoma lung metastasis in chronic alcohol drinking mice
Clinical & experimental metastasis, Vol.28(3), pp.301-307
03/2011
Handle:
https://hdl.handle.net/2376/115348
PMCID: PMC3086720
PMID: 21234656
Abstract
We previously found that chronic alcohol consumption (20% w/v in drinking water) that models the level consumed by human alcoholics, when administered to female C57BL/6 mice inhibits B16BL6 melanoma metastasis to the lung; however, the mechanism is not known. Chronic alcohol consumption increases IFN-γ producing NK, NKT, CD4+, and CD8+ T cells. To examine the impact of IFN-γ on metastasis, we inoculated B16BL6 melanoma cells i.v. into control and chronic alcohol drinking IFN-γ knockout (KO) mice. Knockout of the ifn-γ gene abrogated the anti-metastatic effects associated with alcohol consumption. We examined metastasis in common gamma-chain (γC) KO mice, which are deficient in NK, NKT and CD8+ T cells, and in Vα14Jα281−/− KO mice, which are deficient in invariant NKT (iNKT) cells, in order to assess the importance of specific IFN-γ producing cell types to this effect. We found that the antimetastatic effect of alcohol was still present in γC KO mice and also in γC KO mice depleted of Gr-1+ cells. Knockout of iNKT cells reduced the degree but not the antimetastatic effect associated with alcohol. These results indicate that the antimetastatic effect induced by chronic alcohol consumption is IFN-γ dependent and that multiple IFN-γ producing cell types contribute to this effect.
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Details
- Title
- IFN-γ is essential for the inhibition of B16BL6 melanoma lung metastasis in chronic alcohol drinking mice
- Creators
- Hui Zhang - Department of Pharmaceutical Sciences College of Pharmacy, Washington State University Pullman WA 99164-6534 USAZhaohui Zhu - Department of Pharmaceutical Sciences College of Pharmacy, Washington State University Pullman WA 99164-6534 USAJenifer McKinley - Department of Pharmaceutical Sciences College of Pharmacy, Washington State University Pullman WA 99164-6534 USAGary Meadows - Department of Pharmaceutical Sciences College of Pharmacy, Washington State University Pullman WA 99164-6534 USA
- Publication Details
- Clinical & experimental metastasis, Vol.28(3), pp.301-307
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547799001842
- Language
- English
- Resource Type
- Journal article