Journal article
IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites
Veterinary immunology and immunopathology, Vol.84(3), pp.237-251
2002
Handle:
https://hdl.handle.net/2376/114875
PMID: 11777537
Abstract
The requirement for IFN-γ and/or TNF-α as co-stimulants with
Babesia
bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified
B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN-γ and TNF-α unless the monocytes taken ex vivo were producing TNF-α endogenously. Under the latter condition, the NO production resulting from merozoite stimulation remained IFN-γ-dependent. There was no evidence for endogenous synthesis of TNF-α in monocyte-derived macrophages (MDM), and merozoites alone were incapable of inducing TNF-α mRNA in MDM. However, while merozoites plus IFN-γ induced TNF-α mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes.
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Details
- Title
- IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites
- Creators
- W.L Goff - Animal Disease Research Unit, USDA-ARS, Washington State University, 3003 ADBF, P.O. Box 646630, Pullman, WA 99164-6630, USAW.C Johnson - Animal Disease Research Unit, USDA-ARS, Washington State University, 3003 ADBF, P.O. Box 646630, Pullman, WA 99164-6630, USAS.M Parish - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAG.M Barrington - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAT.H Elsasser - Growth Biology Laboratory, Animal and Nutrition Research Institute, Beltsville Agricultural Research Center, USDA-ARS, Beltsville, MD 20705-2350, USAW.C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USAR.A Valdez - Animal Disease Research Unit, USDA-ARS, Washington State University, 3003 ADBF, P.O. Box 646630, Pullman, WA 99164-6630, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.84(3), pp.237-251
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547996801842
- Language
- English
- Resource Type
- Journal article